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首页> 外文期刊>Environmental toxicology and chemistry >RNA SEQUENCING ANALYSIS OF TRANSCRIPTIONAL CHANGE IN THE FRESHWATER MUSSEL ELLIPTIO COMPLANATA AFTER ENVIRONMENTALLY RELEVANT SODIUM CHLORIDE EXPOSURE
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RNA SEQUENCING ANALYSIS OF TRANSCRIPTIONAL CHANGE IN THE FRESHWATER MUSSEL ELLIPTIO COMPLANATA AFTER ENVIRONMENTALLY RELEVANT SODIUM CHLORIDE EXPOSURE

机译：与环境有关的氯化钠暴露后淡水鹅COMP的转录变化的RNA测序分析

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摘要

To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled sodium chloride (NaCl) exposures. Ribonucleic acid sequencing (RNA-Seq) of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for 7 d, of which 290 had nonoverlapping intervals. Differentially expressed gene categories included ion and transmembrane transport, oxidoreductase activity, maintenance of protein folding, and amino acid metabolism. The rate-limiting enzyme for synthesis of taurine, an amino acid frequently linked to osmotic stress in aquatic species, was upregulated, as was the transmembrane ion pump sodium/potassium adenosine 5'-triphosphatase. These patterns confirm a primary transcriptional response to the experimental dose, albeit likely overlapping with nonspecific secondary stress responses. Substantial involvement of the heat shock protein 70 chaperone family and the water-transporting aquaporin family was not detected, however, in contrast to some studies in other bivalves. A subset of the most significantly regulated genes was confirmed by quantitative polymerase chain reaction in an independent sample. Cluster analysis showed separation of mussels exposed to 2 ppt NaCl from control mussels in multivariate space, but mussels exposed to 1 ppt NaCl were largely indistinguishable from controls. Transcriptome-scale analysis of salt exposure under laboratory conditions efficiently identified candidate biomarkers for further functional analysis and field validation. Published 2017 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.
机译：为了确定淡水前哨物种中盐胁迫的潜在生物标记，我们研究了贻贝常见的贻贝对受控氯化钠（NaCl）的转录反应。地幔组织的核糖核酸测序（RNA-Seq）确定了在暴露于2 ppt NaCl（1.2 ppt氯化物）达7 d的成年贻贝中差异表达的481个转录物，其中290个具有非重叠间隔。差异表达的基因类别包括离子和跨膜转运，氧化还原酶活性，蛋白质折叠的维持以及氨基酸代谢。牛磺酸（一种经常与水生物种中的渗透压相关的氨基酸）的合成限速酶和跨膜离子泵钠/钾腺苷5'-三磷酸酶也被上调。这些模式证实了对实验剂量的初级转录反应，尽管可能与非特异性次级应激反应重叠。然而，与其他双壳类动物的一些研究相比，没有发现热激蛋白70伴侣家族和水传输水通道蛋白家族的实质性参与。在独立样品中通过定量聚合酶链反应确认了调节最明显的基因的子集。聚类分析表明，在多变量空间中，暴露于2 ppt NaCl的贻贝与对照贻贝的分离，但暴露于1 ppt NaCl的贻贝与对照在很大程度上没有区别。在实验室条件下对盐分的转录组规模分析有效地鉴定了候选生物标志物，用于进一步的功能分析和现场验证。代表SETAC发布2017年Wiley Periodicals Inc.。本文是美国政府的工作，因此，它属于美国的公共领域。

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