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首页> 外文期刊>Environmental toxicology and chemistry >DETECTION OF RETINOIC ACID RECEPTOR AGONISTIC ACTIVITY AND IDENTIFICATION OF CAUSATIVE COMPOUNDS IN MUNICIPAL WASTEWATER TREATMENT PLANTS IN JAPAN
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DETECTION OF RETINOIC ACID RECEPTOR AGONISTIC ACTIVITY AND IDENTIFICATION OF CAUSATIVE COMPOUNDS IN MUNICIPAL WASTEWATER TREATMENT PLANTS IN JAPAN

机译：日本城市污水处理厂中维甲酸受体的活性检测及致病性化合物的鉴定

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摘要

Abstract Retinoic acid (RA) receptor (RAR) agonists are potential toxicants that can cause teratogenesis in vertebrates. To determine the occurrence of RAR agonists in municipal wastewater treatment plants (WWTPs), we examined the RARa agonistic activities of influent and effluent samples from several municipal WWTPs in Osaka, Japan, using a yeast two-hybrid assay. Significant RARa agonistic activity was delected in all the influent samples investigated, suggesting that municipal wastewater consistently contains RAR agonists. Fractionations using high-performance liquid chromatography, directed by the bioassay, found several bioactive peaks from influent samples. The RAR agonists. all-trans RA (atRA), 13-cis RA (13cRA), 4-oxo-atRA, and 4-oxo-13cRA, possibly arising from human urine, were identified by liquid chromatography ion trap time-of-flight mass spectrometry. Quantification of the identified compounds in municipal WWTPs confirmed that they were responsible for the majority of RARα agonistic activity in WWTP influents, and also revealed they were readily removed from wastewater by activated sludge treatment. Simultaneous measurement of the RARa agonistic activity revealed that although total activity typically declined concomitant with the reduction of the four identified compounds, it remained high after the decline of RAs and 4-oxo-RAs in one WWTP, suggesting the occurrence of unidentified RAR agonists during the activated sludge treatment.
机译：摘要维甲酸（RA）受体（RAR）激动剂是可能导致脊椎动物致畸的潜在毒物。为了确定RAR激动剂在市政废水处理厂（WWTP）中的发生，我们使用酵母两杂交法检测了日本大阪市多个市政WWTP的进水和出水样品的RARa激动活性。在所有调查的进水样品中均检测到了显着的RARa激动活性，表明市政废水始终含有RAR激动剂。在生物测定的指导下，使用高效液相色谱法进行分离，从进水样品中发现了几个生物活性峰。 RAR激动剂。通过液相色谱离子阱飞行时间质谱法鉴定了可能来自人尿的全反式RA（atRA），13-顺式RA（13cRA），4-oxo-atRA和4-oxo-13cRA。对市政污水处理厂中所鉴定化合物的定量证实，它们是污水处理厂进水中大部分RARα激动活性的原因，并且还表明通过活性污泥处理可轻松将其从废水中去除。同时测量RARa激动活性的方法显示，尽管总活性通常随四种已鉴定化合物的减少而下降，但在一个污水处理厂中RA和4-oxo-RA下降后，其总活性仍然很高，这表明在此期间出现了未确定的RAR激动剂。活性污泥处理。

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  • 来源
    《Environmental toxicology and chemistry》 |2012年第2期|p.307-315|共9页
  • 作者

    Kazuko Sawada; Daisuke Inoue; Yuichiro Wada; Kazunari Sei; Tsuyoshi Nakanishi; Michihiko Ike;

  • 作者单位

    Division of Sustainable Energy and Environmental Engineering, Osaka University, Suita, Osaka, Japan;

    Division of Sustainable Energy and Environmental Engineering, Osaka University, Suita, Osaka, Japan;

    Division of Sustainable Energy and Environmental Engineering, Osaka University, Suita, Osaka, Japan;

    Division of Sustainable Energy and Environmental Engineering, Osaka University, Suita, Osaka, Japan;

    Laboratory of Hygienic- Chemistry and Molecular Toxicology, Gifu Pharmaceutical University, Gifu, Japan;

    Division of Sustainable Energy and Environmental Engineering, Osaka University, Suita, Osaka, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    retinoic acid receptor; retinoic acid receptor agonist; wastewatcr treatment plant; retinoic acid; 4-oxo-relinoic acid;

    机译：视黄酸受体视黄酸受体激动剂废水处理厂;视黄酸4-氧-松香酸;

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