...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >EFFECTS OF THE PEROXISOME PROLIFERATOR DI(2-ETHYLHEXYL)PHTHALATE ON CELL TURNOVER AND PEROXISOME PROLIFERATION IN PRIMARY CHICK EMBRYO HEPATOCYTES
【24h】

EFFECTS OF THE PEROXISOME PROLIFERATOR DI(2-ETHYLHEXYL)PHTHALATE ON CELL TURNOVER AND PEROXISOME PROLIFERATION IN PRIMARY CHICK EMBRYO HEPATOCYTES

机译：过氧化物酶体增殖物二（2-乙基己基）邻苯二甲酸酯对小鸡胚肝细胞周转率和过氧化物酶体增殖的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The peroxisome proliferator (PP) di(2-ethylhexyl)phthalate (DEHP) is widely used as a plasticizer and can contaminate air, water, and soil. As yet, no data have been published on its potential to induce changes in cell growth of nonmammalian hepatocytes. In the present study, the effects of DEHP on cell turnover and induction of peroxisome proliferation were evaluated in primary hepatocyte cultures from chick embryos. Cells were treated after attachment with 0,25,50,75, and 100 μM DEHP for up to 96 h. S-phase increased significantly (p < 0.01) from a background level of 5.5 ± 0.1 % in solvent-control hepatocytes to a maximum level of 7.1 ± 0.1 % in cells exposed for 48 h to 100 μM DEHP and decreased to near 6% by 96 h. Lower (p < 0.05) levels of induction were seen at 50 and 75 μM DEHP. Spontaneous apoptosis showed a slight (p < 0.05) decrease in hepatocytes treated with ≥75 μM dosages, as measured at 72 to 96 h. Induction of peroxisome proliferation was observed for cultures treated with ≥75 μM dosages at 48 h onwards. The results of the present study indicate that avian species may be responsive to the effects of PPs and may thus be affected by the presence of DEHP in the environment, but that this species is less sensitive than rodents.
机译：过氧化物酶体增殖物（PP）邻苯二甲酸二（2-乙基己基）酯（DEHP）被广泛用作增塑剂，可污染空气，水和土壤。迄今为止，尚未有关于其诱导非哺乳动物肝细胞生长变化的潜能的数据。在本研究中，在雏鸡胚胎的原代肝细胞培养物中评估了DEHP对细胞更新和过氧化物酶体增殖的影响。附着后，用0、25、50、75和100μMDEHP处理细胞长达96小时。 S期从溶剂控制肝细胞中的背景水平5.5±0.1％显着增加（p <0.01）至暴露于100μMDEHP 48 h的细胞中的最高水平7.1±0.1％，并在降低至6％的情况下降低96小时在50和75μMDEHP下观察到较低的诱导水平（p <0.05）。在72到96小时测量，用≥75μM剂量处理的肝细胞自发凋亡显示轻微（p <0.05）减少。从48小时起，用≥75μM剂量处理的培养物观察到过氧化物酶体增殖的诱导。本研究的结果表明，禽类可能对PP的反应有反应，因此可能受到环境中DEHP的影响，但该物种不如啮齿动物敏感。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号