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首页> 外文期刊>Environmental toxicology and chemistry >VITELLOGENIN INDUCTION BY 17β-ESTRADIOL AND 17α-ETHYNYLESTRADIOL IN MALE MURRAY RAINBOWFISH {MELANOTAENIA FLUVIATILIS)
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VITELLOGENIN INDUCTION BY 17β-ESTRADIOL AND 17α-ETHYNYLESTRADIOL IN MALE MURRAY RAINBOWFISH {MELANOTAENIA FLUVIATILIS)

机译：17β-雌二醇和17α-乙炔雌二醇在男性墨鱼花粉中的卵黄素诱导表达（黄褐变）

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摘要

Hepatic vitellogenin (VTG) mRNA and plasma protein assays were developed for the Murray rainbowfish (Melanotaenia fluviatilis), a native freshwater fish species in Australia. Adult male Murray rainbowfish were exposed to 17β-estradiol (E2) or 17α-ethynylestradiol (EE2) at 0, 1, 5, 10, 50, or 100ng/L in a semistatic system for 7d. Vitellogenin mRNA was quantified by quantitative polymerase chain reaction, and VTG protein was semiquantified in plasma using an enzyme-linked immunosorbent assay. Water concentrations were quantified by liquid chromatography coupled with tandem mass spectrometry and were on average 110% and 85% nominal concentrations for E2 and EE2, respectively. Vitellogenin transcripts and protein were upregulated in male Murray rainbowfish exposed to either E2 or EE2 in a dose-responsive manner, with calculated 10% effective concentration (EC10) values for E2 of 3.71 ng/L and 11.6 ng/L for VTG mRNA and protein, respectively, and for EE2 of 2.77 ng/L and 8.47 ng/L for VTG mRNA and protein, respectively. Comparisons of these responses with responses of commonly used test species, including zebrafish (Danio rerio), fathead minnow (Pimephales promelas), and Japanese medaka (Oryzias latipes), revealed the Murray rainbowfish to be a sensitive test species for estrogenic effects via the estrogen receptor pathway. The present study suggests that the Murray rainbowfish would be a suitable candidate for future field studies designed for assessing estrogenic effects of effluent discharges in the Australian freshwater environment.
机译：肝卵黄蛋白原（VTG）mRNA和血浆蛋白测定法是针对墨累彩虹鱼（Melanotaenia fluviatilis）开发的，该墨鱼是澳大利亚的一种淡水鱼类。成年雄性Murray虹鱼在半静态系统中以0、1、5、10、50或100ng / L的浓度暴露于17β-雌二醇（E2）或17α-乙炔基雌二醇（EE2）7d。通过定量聚合酶链反应对卵黄蛋白原mRNA进行定量，并使用酶联免疫吸附法对血浆中的VTG蛋白进行半定量。通过液相色谱和串联质谱法对水浓度进行定量，E2和EE2的平均标称浓度分别为110％和85％。暴露于E2或EE2的雄性Murray虹鱼中的卵黄蛋白原转录物和蛋白呈剂量响应方式上调，计算得出E2的10％有效浓度（EC10）值为3.71 ng / L，VTG mRNA和蛋白为11.6 ng / L EE2的VTG mRNA和蛋白质分别为2.77 ng / L和8.47 ng / L。将这些响应与斑马鱼（Danio rerio），黑头min鱼（Pimephales promelas）和日本（Oryzias latipes）等常用测试物种的响应进行比较，发现墨累彩虹鱼是通过雌激素产生雌激素作用的敏感测试物种。受体途径。本研究表明，墨累彩虹鱼将是未来野外研究的合适人选，该野外研究旨在评估澳大利亚淡水环境中废水排放的雌激素作用。

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