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首页> 外文期刊>Environmental toxicology and chemistry >EVALUATION OF ESTROGENICITY OF SEWAGE EFFLUENT AND RECLAIMED WATER USING VITELLOGENIN AS A BIOMARKER
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EVALUATION OF ESTROGENICITY OF SEWAGE EFFLUENT AND RECLAIMED WATER USING VITELLOGENIN AS A BIOMARKER

机译：以Vitlogenin为生物标志物评价污水和再生水的雌激素性。

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To evaluate the quality of reclaimed water, the estrogenicity of effluent from a sewage treatment plant and of reclaimed water treated with coagulation-sedimentation-filtration using the effluent as raw water was assessed using vitellogenin (VTG) as a biomarker. After a three-week exposure, significant (p < 0.05) induction of VTG occurred in female crucian carp (Carassius carassius) exposed continuously to the secondary effluent and reclaimed water with different dilutions (12.5, 25, 50, and 100% for secondary effluent; 50 and 100% for reclaimed water); no induction of VTG was detected when exposed to 12.5 and 25% reclaimed water. For male fish, however, only 100% secondary effluent induced the production of VTG (mean ± standard deviation, 38.6 ± 9.8 μg/ml). When the exposure time was prolonged to three months, VTG was induced significantly in both females and males at all gradient concentrations of secondary effluent and at 50 and 100% reclaimed water. The results indicated that no obvious VTG was detected in fish exposed to reclaimed water diluted more than fourfold. Ozonation of the secondary effluent under an ozone consumption dose of 8.5 mg/L resulted in a VTG level equal to that of 12.5% secondary effluent or 50% reclaimed water. Furthermore, VTG induction reflects the cumulative effects of estrogenic activity in the secondary effluent and reclaimed water compared with the in vitro assays, in which estrogenic activities in effluent changed markedly during the experiment.
机译：为了评估再生水的质量，使用卵黄蛋白原（VTG）作为生物标记物，评估了污水处理厂的废水和经凝结沉淀过滤处理后的再生水（使用废水作为原水）的雌激素性。暴露三周后，连续暴露于次级污水和不同稀释度（次级污水分别为12.5％，25％，50％和100％）的再生水的female鱼（Carassius carassius）发生了显着（p <0.05）的VTG诱导。 ；再生水的50％和100％）；当暴露于12.5％和25％的再生水中时，未检测到VTG的诱导。但是，对于公鱼，只有100％的二次流出物诱导了VTG的产生（平均值±标准偏差，38.6±9.8μg/ ml）。当暴露时间延长至三个月时，在次要废水的所有梯度浓度下以及再生水分别为50％和100％时，在雌性和雄性中均显着诱导了VTG。结果表明，暴露于稀释了四倍以上的再生水的鱼中未检测到明显的VTG。在8.5 mg / L的臭氧消耗剂量下对次级废水进行臭氧处理，其VTG含量等于次级废水的12.5％或再生水的50％。此外，与体外试验相比，VTG诱导反应反映了次级废水和再生水中雌激素活性的累积作用，在体外试验中，废水中的雌激素活性发生了显着变化。

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