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首页> 外文期刊>Environmental toxicology and chemistry >OXIDATIVE DAMAGE IN UNFERTILIZED EGGS OF CHINESE RARE MINNOW (GOBIOCYPRIS RARUS) EXPOSED TO NONYLPHENOL
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OXIDATIVE DAMAGE IN UNFERTILIZED EGGS OF CHINESE RARE MINNOW (GOBIOCYPRIS RARUS) EXPOSED TO NONYLPHENOL

机译：暴露于壬基酚的中国稀有小食（GOBIOCYPRIS RARUS）未生产的鸡蛋中的氧化性损伤

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摘要

In the present study, female Chinese rare minnows (Gobiocypris rarus) were used as in vivo models and exposed to nonylphenol (NP) at concentrations of 1 to 200 (μg/L for 21 d under semistatic conditions. Molecular biomarkers of oxidative stress were measured in unfertilized eggs and included reactive oxygen species (ROS), lipid peroxidation products (thiobarbituric acidreactive substances [TEARS] and protein carbonyl), superoxide dismutase activity, and glutathione. Cathepsin D activity as an indicator of egg viability also was assayed. Nonylphenol induced ROS formation in unfertilized eggs in all exposed groups compared to the controls. The levels of protein carbonyl and TBARS in unfertilized eggs were significantly increased (p < 0.05) at 10 to 200 and 100 to 200 μg/L, respectively. Good positive correlations were shown between ROS induction and levels of TBARS and protein carbonyl in eggs (R = 0.918, p < 0.05 and R = 0.784, p < 0.05, respectively). Superoxide dismutase activity in eggs was significantly inhibited (p < 0.05) in the 50 to 200 μg/L exposure groups. Glutathione levels in eggs were significantly depleted (p < 0.05) at 100 to 200 μg/L concentrations. In addition, ROS induction resulted in oxidative damage to lipid and protein in chorions. Significant reductions (p < 0.05) of the protein and lipid contents in chorions were both found in the 50 to 200 μg/L exposure groups. A previous study found that NP exposure could lead to chorion thinning in zebra fish. Thus, the reductions in protein and lipid contents in chorion could be the reason for chorion thinning by NP exposure. Meanwhile, cathepsin D activity was significantly inhibited (p < 0.05) in all exposure groups. The results demonstrated that NP-induced oxidative stress could damage the chorion of unfertilized eggs and lead to a decline in gamete quality in female Chinese rare minnow.
机译：在本研究中，以雌性中国小min鱼（Gobiocypris rarus）作为体内模型，并在半静态条件下以1-200（μg/ L）的浓度暴露于壬基酚（NP）21天，测定了氧化应激的分子生物标志物。在未受精卵中，包括活性氧（ROS），脂质过氧化产物（硫代巴比妥酸反应性物质[TEARS]和蛋白羰基），超氧化物歧化酶活性和谷胱甘肽，还测定了组织蛋白酶D活性作为卵生存力的指标。与对照组相比，所有暴露组的未受精卵中的蛋白质形成量均显着升高，未受精卵中的羰基蛋白和TBARS水平分别在10至200和100至200μg/ L时显着增加（p <0.05），并显示出良好的正相关性。 ROS诱导与鸡蛋中TBARS和蛋白质羰基水平的关系（分别为R = 0.918，p <0.05和R = 0.784，p <0.05）在50至200μg/ L的暴露组中，ggs被显着抑制（p <0.05）。在100至200μg/ L的浓度下，鸡蛋中的谷胱甘肽水平显着减少（p <0.05）。另外，ROS的诱导导致绒毛膜中脂质和蛋白质的氧化损伤。在50至200μg/ L的暴露组中，绒毛膜中的蛋白质和脂质含量均显着降低（p <0.05）。先前的研究发现，NP暴露可能导致斑马鱼的绒毛膜变薄。因此，绒毛膜中蛋白质和脂质含量的降低可能是NP暴露导致绒毛膜变薄的原因。同时，在所有暴露组中组织蛋白酶D活性均被显着抑制（p <0.05）。结果表明，NP诱导的氧化应激可能损害未受精卵的绒毛膜，并导致雌性中国稀有min鱼的配子质量下降。

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