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首页> 外文期刊>Environmental toxicology and chemistry >ARSENATE-INDUCED TOXICITY: EFFECTS ON ANTIOXIDATIVE ENZYMES AND DNA DAMAGE IN VICIA FABA
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ARSENATE-INDUCED TOXICITY: EFFECTS ON ANTIOXIDATIVE ENZYMES AND DNA DAMAGE IN VICIA FABA

机译：砷酸诱导的毒性：对蚕豆中抗氧化酶和DNA损伤的影响

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摘要

A glasshouse hydroponic experiment was conducted to investigate the toxicity of arsenate to broad bean (Vicia faba) plants when grown with external arsenate concentrations at 10 μmol/L. The treated plants showed no obvious symptoms of phytotoxicity, but shoot/root growth was inhibited. Lipid peroxidation in leaves and roots increased with the addition of arsenate, indicating oxidative stress. We investigated the responses to arsenate exposure in the activities of several representative antioxidant enzymes, including peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), in plant tissues, and the DNA damage in plant leaves and root tips induced by arsenate in this plant was detected for the first time. Arsenate addition increased POD activity in the leaves significantly but decreased its activity in the roots in the 10 μmol/L treatment. Arsenate addition caused an induction of SOD and CAT activities in both leaves and roots, but not in roots at the arsenate concentration of 10 μmol/L. The DNA damage in V. faba was detected using Comet assay; in both leaves and roots, DNA damage increased with increasing arsenate concentrations, indicating genotoxicity of arsenate. These results indicate that arsenate toxicity causes oxidative stress in V. faba, which might be one of the mechanisms through which arsenic induces DNA damage.
机译：进行了温室水培试验，以研究砷在外部浓度为10μmol/ L的条件下对蚕豆（蚕豆）的毒性。处理过的植物没有明显的植物毒性症状，但是芽/根的生长受到抑制。叶和根中脂质的过氧化随着砷酸的添加而增加，表明氧化应激。我们调查了植物组织中几种代表性抗氧化剂酶（包括过氧化物酶（POD），过氧化氢酶（CAT）和超氧化物歧化酶（SOD））对砷暴露的响应，以及由诱导的植物叶和根尖中的DNA损伤。首次检测到该植物中的砷酸盐。在10μmol/ L处理中，添加砷酸盐显着增加了叶片中的POD活性，但降低了其在根中的活性。砷浓度为10μmol/ L时，在叶片和根中都诱导了SOD和CAT的活性，但在根中却没有。使用彗星分析法检测了蚕豆中的DNA损伤。在叶片和根部，DNA损伤都随着砷酸盐浓度的增加而增加，表明砷酸盐的遗传毒性。这些结果表明砷酸盐的毒性导致蚕豆中的氧化应激，这可能是砷诱导DNA损伤的机制之一。

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  • 来源
    《Environmental toxicology and chemistry》 |2008年第2期|413-419|共7页
  • 作者

    Aijun Lin; Xuhong Zhang; Yong-Guan Zhu; Fang-Jie Zhao;

  • 作者单位

    Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029,People's Republic of ChinarnState Key Lab of Regional and Urban Ecology/Department of Soil Environmental Sciences, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China;

    Beijing City University, Beijing 100083, People's Republic of China;

    State Key Lab of Regional and Urban Ecology/Department of Soil Environmental Sciences, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361003, People's Republic of China;

    Agriculture and the Environment Division, Rothamsted Research, Harpenden, Herts AL5 2JQ, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    arsenate; vicia faba; oxidative stress; DNA damage;

    机译：砷蚕豆氧化应激DNA损伤;

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