...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >Dna Microarrays Detect Effects Of Soil Contamination On Arabidopsis Thaliana Gene Expression
【24h】

Dna Microarrays Detect Effects Of Soil Contamination On Arabidopsis Thaliana Gene Expression

机译：DNA芯片检测土壤污染对拟南芥拟南芥基因表达的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Soil contamination, such as heavy metals and benzene compounds, is a widespread problem on military installations. It is important to be able to determine the effects of soil contamination before any adverse effects appear in organisms in surrounding areas. We examined gene expression in Arabidopsis thaliana grown in soil from three sites at the Radford Army Ammunition Plant in Radford, Virginia, USA, using DNA microarrays. We analyzed soil, germination, and growth rate to compare with the microarray data. Soil contamination affected both external phenotype and gene expression. Plants grown in soil with high levels of contaminants were chloritic and were smaller than control plants grown in potting soil. Plants grown in soil with the highest copper concentration had the lowest growth rates and had genes up-regulated across several functional groups. Plants grown in soils with elevated lead had many genes down-regulated that were related to photosystem II, metabolism, cellular transport, and protein synthesis. Genes consistently up-regulated across most microarrays were genes related to photosystem I, genes related to water deprivation and oxidative stress response, heat shock proteins, and toxin catabolism genes such as glutathiones. DNA microarrays, in concert with a model genetic organism such as A. thaliana, were an effective assessment tool to determine the presence of toxic substances in soil at a site used for the production of military explosives.
机译：重金属和苯化合物等土壤污染是军事设施中普遍存在的问题。重要的是要能够确定土壤污染的影响，然后才能对周围地区的生物造成任何不利影响。我们使用DNA微阵列芯片检查了美国弗吉尼亚州拉德福德市拉德福德陆军弹药厂三个地方土壤中生长的拟南芥中的基因表达。我们分析了土壤，发芽和生长速率，以与微阵列数据进行比较。土壤污染影响外部表型和基因表达。在土壤中污染物含量高的土壤中生长的植物具有氯性，并且比盆栽土壤中的对照植物小。在铜浓度最高的土壤中生长的植物生长速率最低，并且多个功能组的基因上调。在铅含量升高的土壤中生长的植物的许多基因均下调，这些基因与光系统II，新陈代谢，细胞转运和蛋白质合成有关。在大多数微阵列中一致上调的基因是与光系统I相关的基因，与水分缺乏和氧化应激反应相关的基因，热休克蛋白和毒素分解代谢基因（如谷胱甘肽）。 DNA芯片与典型遗传生物（如拟南芥）协同作用，是一种有效的评估工具，可用于确定用于生产军事炸药的土壤中是否存在有毒物质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号