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首页> 外文期刊>Environmental toxicology and chemistry >BIOMONITORING OF ESTROGENIC EXPOSURE AND IDENTIFICATION OF RESPONSIBLE COMPOUNDS IN BREAM FROM DUTCH SURFACE WATERS
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BIOMONITORING OF ESTROGENIC EXPOSURE AND IDENTIFICATION OF RESPONSIBLE COMPOUNDS IN BREAM FROM DUTCH SURFACE WATERS

机译：荷兰表层水域中性激素暴露的生物监测和负责任化合物的鉴定

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The exposure to and effects of estrogenic compounds in male breams from Dutch freshwater locations were investigated. Ovotestis was observed infrequently (maximum frequency 16%). However, plasma vitellogenin (VTG) concentration was elevated highly at some locations. Estrogenic activities in male bream plasma, liver, and in gastrointestinal content were measured in the estrogen-responsive chemical-activated luciferase gene expression (ER-CALUX~®) assay. Plasma concentrations of vitellogenin correlated very well with the estrogenic activities in gastrointestinal content. The ER-CALUX activity in gastrointestinal content thus could provide a biomarker for recent exposure to estrogenic compounds, and the gastrointestinal content was chosen as investigative matrix for the toxicity identification and evaluation ([TIE]; bioassay-directed fractionation) of estrogenic compounds in bream. The approach consisted of a reversed-phase high-performance liquid chromatography fractionation of gastrointestinal content extract, directed by ER-CALUX and followed by gas chromatography analysis. The estrogenic hormones 17β-estradiol and its metabolite estrone were identified as major contributors to the activity at all locations (except the reference location), independent of the presence or absence of a known source of estrogenic activity, such as a sewage treatment plant. Chemical screening showed the presence of other pollutants, such as a lower chlorinated dioxin and the disinfectants clorophene and triclosan. However, these compounds did not have high estrogenic potencies and their concentrations were not high enough to contribute significantly to the observed estrogenic activity.
机译：对来自荷兰淡水地点的雄性to中雌激素化合物的暴露及其影响进行了研究。很少观察到卵睾丸（最大频率为16％）。但是，血浆卵黄蛋白原（VTG）的浓​​度在某些位置明显升高。通过雌激素反应性化学激活的萤光素酶基因表达（ER-CALUX®）测定来测量雄性鲷血浆，肝脏和胃肠道中的雌激素活性。卵黄蛋白原的血浆浓度与胃肠道内容物中的雌激素活性非常相关。因此，胃肠道内容物中的ER-CALUX活性可以为最近暴露于雌激素化合物提供生物标记，并选择胃肠道内容物作为鲷鱼中雌激素化合物的毒性鉴定和评估（[TIE]；生物测定定向分级）的研究基质。 。该方法包括在ER-CALUX的指导下对胃肠道内容物提取物进行反相高效液相色谱分馏，然后进行气相色谱分析。雌激素17β-雌二醇及其代谢物雌酮被确定为所有位置（参考位置除外）活动的主要贡献者，而与是否存在已知雌激素活性来源（如污水处理厂）无关。化学筛选显示存在其他污染物，例如较低的氯代二恶英和消毒剂克罗芬和三氯生。然而，这些化合物不具有高的雌激素效力，并且它们的浓度还不足以显着地促进所观察到的雌激素活性。

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