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首页> 外文期刊>Environmental toxicology and chemistry >BIOCHEMICAL AND SPECTROSCOPIC STUDIES OF THE RESPONSE OF CONVOLVULUS ARVENSIS L. TO CHROMIUM(Ⅲ) AND CHROMIUM(Ⅵ) STRESS
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BIOCHEMICAL AND SPECTROSCOPIC STUDIES OF THE RESPONSE OF CONVOLVULUS ARVENSIS L. TO CHROMIUM(Ⅲ) AND CHROMIUM(Ⅵ) STRESS

机译:卷积小菜对铬(Ⅲ)和铬(Ⅵ)胁迫反应的生物化学和光谱学研究

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摘要

The objective of the present study was to determine the oxidative stress caused by hexavalent chromium (Cr[Ⅵ]), the chromium (Cr) uptake, and the Cr speciation in Convolvulus arvensis L. plants grown in hydroponics media containing either Cr(Ⅵ) or Cr(Ⅲ). The results demonstrated that C. arvensis plants exposed to Cr(Ⅵ) concentrations ranging from 0 to 40 mg/L expressed higher ascorbate peroxidase specific activity in roots than in shoots. On the other hand, catalase activity monitored in plants exposed to 2 mg/L of Cr(Ⅵ) for 24 h increased in roots after a few hours of exposure. However, catalase activity in shoots revealed a decrement almost immediately after treatment was initiated. The results from x-ray absorption spectroscopic studies indicated that the oxidation state of the supplied Cr(Ⅲ) remained the same in plant tissues. The supplied Cr(Ⅵ), however, was reduced to the trivalent form in plant tissues. The results of inductively coupled plasma/optical emission spectroscopy demonstrated that after 5 d, the roots of plants exposed to 40 mg/L of Cr(Ⅲ) or Cr(Ⅵ) accumulated approximately 25,000 and 3,500 mg/kg dry weight of Cr, respectively. Nevertheless, shoots concentrated 1,500 and 2,000 mg/kg dry weight of Cr from Cr(Ⅲ) and Cr(Ⅵ), respectively, which indicated that Cr moved faster into C. arvensis plants when supplied as Cr(Ⅵ).
机译:本研究的目的是确定在六价铬(Cr [Ⅵ])种植在含有Cr(Ⅵ)的水培培养基中的空心菜中的六价铬(Cr [Ⅵ])引起的氧化胁迫,铬(Cr)吸收和Cr形态形成。或Cr(Ⅲ)。结果表明,暴露于Cr(Ⅵ)浓度为0至40 mg / L的C. arvensis植物的根部抗坏血酸过氧化物酶比活性高于芽。另一方面,暴露于几个小时后,在暴露于2 mg / L Cr(Ⅵ)24小时的植物中监测到的过氧化氢酶活性在根部增加。然而,芽中的过氧化氢酶活性几乎在治疗开始后立即下降。 X射线吸收光谱研究的结果表明,植物组织中所提供的Cr(Ⅲ)的氧化态保持不变。但是,提供的Cr(Ⅵ)在植物组织中被还原成三价形式。电感耦合等离子体/光发射光谱法的结果表明,5 d后,暴露于40 mg / L Cr(Ⅲ)或Cr(Ⅵ)的植物的根部分别累积了约25,000和3500 mg / kg的干重。 。然而,新梢分别从Cr(Ⅲ)和Cr(Ⅵ)中浓缩了1,500和2,000 mg / kg干重的Cr,这表明当以Cr(Ⅵ)形式供应时,Cr更快地运入C. arvensis植物。

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