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首页> 外文期刊>Environmental toxicology and chemistry >INDUCTION OF VITELLOGENIN mRNA IN JUVENILE CHINESE STURGEON (ACIPENSER SINENSIS GRAY) TREATED WITH 17β-ESTRADIOL AND 4-NONYLPHENOL
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INDUCTION OF VITELLOGENIN mRNA IN JUVENILE CHINESE STURGEON (ACIPENSER SINENSIS GRAY) TREATED WITH 17β-ESTRADIOL AND 4-NONYLPHENOL

机译：17β-雌二醇和4-壬基酚处理的少年中华ST中的卵黄素mRNA的诱导

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摘要

It has been demonstrated that 4-nonylphenol (4-NP) exerts estrogenic effects in diverse fishes. The present study investigated the effects of 4-NP on Chinese sturgeon (Acipenser sinensis Gray) vitellogenin (VTG) gene expression. By reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers, a 462-base pair fragment of Chinese sturgeon VTG, corresponding to a 154-amino acid sequence, was amplified and sequenced. This sequence exhibited 152/154 identity to the amino acid sequence of white sturgeon (A. transmontano) VTG. Conventional RT-PCR and quantitative real-time RT-PCR were established and used to study the VTG and β-actin gene expression in the liver of juvenile Chinese sturgeon injected three times with 17β-estradiol (5 mg/kg body wt/week) or 4-NP (10 or 100 mg/kg/week) in the course of three weeks. Significant induction of VTG gene expression was detected in all treated groups, and no VTG mRNA was detected in the control group. The ratio of VTG to β-actin analyzed from the results of quantitative real-time RT-PCR reached 0.041 ± 0.024 (mean ± SD) in the group receiving 10 mg/kg/week of 4-NP and 4.51 ± 1.68 in the group receiving 100 mg/kg/week of 4-NP. Chemical analysis of 4-NP showed that the concentrations of 4-NP in the 10 mg/kg/week group and the 100 mg/kg/week group were 2.78 ± 2.41 and 31.38 ± 0,26 μg/ g wet weight, respectively. Compared with the 4-NP concentrations (0.8-1.92 μg/g wet wt) in fish from the Yangtze River, China, a potential hazard exists regarding 4-NP in Chinese sturgeon. These results represent the first indication of the risk of endocrine-disrupting chemicals for Chinese sturgeon.
机译：已经证明4-壬基苯酚（4-NP）在多种鱼类中都具有雌激素作用。本研究调查了4-NP对中华st（Acipenser sinensis Gray）卵黄蛋白原（VTG）基因表达的影响。通过使用简并引物的逆转录-聚合酶链反应（RT-PCR），对Chinese鱼VTG的一个462个碱基对的片段进行扩增和测序，该片段对应于154个氨基酸序列。该序列与白色st鱼（A. transmontano）VTG的氨基酸序列具有152/154的同一性。建立了常规RT-PCR和定量实时RT-PCR方法，用于研究3次注射17β-雌二醇（5 mg / kg体重/周）的中华Chinese的肝脏中的VTG和β-actin基因表达。或在三周内服用4-NP（10或100 mg / kg /周）。在所有治疗组中均检测到显着的VTG基因表达诱导，而在对照组中未检测到VTG mRNA。根据实时定量RT-PCR结果分析，VTG与β-肌动蛋白的比率在接受10 mg / kg /周的4-NP的组中达到0.041±0.024（平均值±SD），在该组中达到4.51±1.68接受100 mg / kg /周的4-NP。 4-NP的化学分析显示，10 mg / kg /周组和100 mg / kg /周组中4-NP的湿重分别为2.78±2.41和31.38±0.26μg/ g湿重。与中国长江中鱼类的4-NP浓度（0.8-1.92μg/ g湿重）相比，中国st鱼中的4-NP存在潜在的危害。这些结果代表了对中华st具有内分泌干扰化学物质风险的第一个迹象。

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