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首页> 外文期刊>Environmental toxicology and chemistry >Effects of hydrogen sulfide to vibrio Fischeri, Scenedesmus vacuolatus, and Daphnia magna.
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Effects of hydrogen sulfide to vibrio Fischeri, Scenedesmus vacuolatus, and Daphnia magna.

机译:硫化氢对费氏弧菌,Scenedesmus vacuumlatus和水蚤(Daphnia magna)的影响。

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The effects of hydrogen sulfide (H2S) were tested in three ecotoxicological tests in order to evaluate its confounding potential in assessment of pore water and groundwater toxicity. The luminescent bacteria Vibrio fischeri, the water flea Daphnia magna, and the microalgae Scenedesmus vacuolatus often are part of a biotest battery. A new technique for the synthesis of hydrogen sulfide solutions of defined concentrations using an electrochemical generator instead of sodium sulfide solutions was used. Because hydrogen sulfide is volatile, the loss rate of H2S was studied over time to enable estimation of the mean test concentrations over the whole test duration. Loss rates were calculated to be 13 +/- 6% after 30 min, and 39 +/- 11% and 43 +/- 16% after a 24- and 48-h exposure time, respectively. Sensitivities of the test organisms in terms of median effective concentration (EC50), corrected for the above loss rates, varied from 0.28 to 0.0036 and 0.055 mM for the luminescent bacteria, the crustacea, andthe algae, respectively. A species-sensitivity distribution using EC and mean lethal concentration literature data for marine and freshwater crustaceans and phytoplankton showed a medium sensitivity of the water flea D. magna, though the bacteria V. fischeri and the algae S. vacuolatus were among the least-sensitive group of organisms. This demonstrates that only the algae and the bacteria are easy to use in the assessment of toxicity of matrices with H2S concentrations above 0.06 mM.
机译:为了评估其在评估孔隙水和地下水毒性方面的混杂潜力,在三个生态毒理学测试中测试了硫化氢(H2S)的作用。发光细菌费氏弧菌,跳蚤水蚤(Daphnia magna)和微藻Scenedesmus vacuumlatus通常是生物测试电池的一部分。使用了一种新技术,该技术使用电化学发生器代替硫化钠溶液来合成规定浓度的硫化氢溶液。由于硫化氢是易挥发的,因此随着时间的推移对H2S的损失率进行了研究,从而可以估算整个测试期间的平均测试浓度。损失率经计算在30分钟后为13 +/- 6%,在暴露24小时和48小时后分别为39 +/- 11%和43 +/- 16%。经上述损失率校正的测试生物体的中值有效浓度(EC50)敏感性,对于发光细菌,甲壳类和藻类分别为0.28至0.0036和0.055 mM。使用EC和海洋和淡水甲壳类和浮游植物的平均致死浓度文献数据进行的物种敏感性分布显示,对水蚤D. magna的敏感性中等,尽管细菌V. fischeri和藻类S. vacuumlatus的敏感性最低。生物群。这表明只有藻类和细菌易于用于评估H2S浓度高于0.06 mM的基质的毒性。

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