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首页> 外文期刊>Environmental toxicology and chemistry >A phytoplankton growth assay for routine in situ environmental assessments.
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A phytoplankton growth assay for routine in situ environmental assessments.

机译：用于常规原位环境评估的浮游植物生长测定法。

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摘要

This study proposes an ecologically relevant and cost-effective phytoplankton growth assay for routine in situ toxicity assessments. Assay procedures were developed applying, to the extent possible, the rationale behind the design of standard algal assays. Chlorella vulgaris was selected as test species because it grows well immobilized in alginate beads and has a wide geographic distribution. The performance of the assay in a freshwater system impacted by acid mine drainage demonstrated the suitability of assay chambers and procedures. The test system, made of inexpensive materials, allowed the rapid and easy deployment of the assay. The deployment of extra chambers at reference sites provided the ability to periodically check whether algal growth had already reached recommended growth criteria (time at which the assay should end). By deploying chambers filled with control medium at all sites, temperature was identified to explain 95% of the variation in growth. By using an artificial nutrient source shown capable of promoting algal growth according to recommended standards, toxicity from the mine effluent was distinguish from in situ nutrient limitation effects. The very good agreement (r2 = 90%) between mean in situ growth rates estimated by microscopy and by spectrophotometry and their similar coefficient of variation showed the latter to be a suitable straightforward methodology for assay endpoint estimation.
机译：这项研究提出了一种生态上相关且具有成本效益的浮游植物生长测定法，用于常规原位毒性评估。根据标准藻类试验设计的基本原理，开发了试验程序。选择小球藻作为测试物种，因为它能很好地固定在藻酸盐珠中，并且具有广泛的地理分布。在酸性矿井排水影响下的淡水系统中，测定的性能证明了测定室和程序的适用性。该测试系统由廉价的材料制成，可以快速，轻松地进行测定。在参考位置部署额外的腔室提供了定期检查藻类生长是否已达到建议的生长标准（测定应终止的时间）的能力。通过在所有位置部署装有控制介质的小室，可以确定温度以解释生长变化的95％。根据推荐标准，使用显示出能够促进藻类生长的人工营养物来源，将矿山废水中的毒性与原位营养物限制作用区分开来。用显微镜和分光光度法估算的平均原位生长速率及其相似的变异系数之间具有很好的一致性（r2 = 90％），表明后者是用于测定终点估算的合适直接方法。

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