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首页> 外文期刊>Environmental toxicology and chemistry >EROD INDUCTION IN CULTURED CHICK EMBRYO LIVER: A SENSITIVE BIOASSAY FOR DIOXIN-LIKE ENVIRONMENTAL POLLUTANTS
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EROD INDUCTION IN CULTURED CHICK EMBRYO LIVER: A SENSITIVE BIOASSAY FOR DIOXIN-LIKE ENVIRONMENTAL POLLUTANTS

机译：培养的鸡胚肝中的腐蚀诱导：对类似二恶英类环境污染物的灵敏生物测定

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摘要

A technique for studying 7-ethoxyresorufin O-deethylase (EROD) induction in chick embryo liver in vitro was developed. Livers from 8-d-old embryos were cultured in rotating vials at 37℃ for 48 h in a medium to which DMSO-dissolved test compounds had been added. This bioassay proved to be highly sensitive to dioxin-like compounds, and its usefulness for assessing the toxic potency of such compounds in environmental samples was demonstrated. Concentration-response curves were determined for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3′,4,4′,5-pentachlorobiphenyl (PCB IUPAC no. 126), 3,3′,4,4′-tetrachlorobiphenyl (PCB 77), 2,3,3′,4,4′-pentachlorobiphenyl (PCB 105), and benzo[k]fluoranthene (BkF). TCDD induced EROD in a concentration-dependent manner, having an EC50 of 5.0 x 10~(-12) M. The cultured embryo livers were extremely sensitive to TCDD, and about 30 fg of this compound per liver (2 x 10~(-13) M) was enough to significantly induce EROD. The EC50 values obtained for PCBs 126, 77, 105, and BkF were 4.4 X 10~(-11) M, 9.2 x 10~(-9) M, 1.6 x 10~(-8) M, and 6.2 x 10~(-7) M, respectively. The maximal induction levels obtained for three different preparations of polychlorinated naphthalenes (PCNs) were less than those of the other compounds tested. When the technical PCN mixture Halowax 1014 was coadministered with TCDD, the induction was lower than that caused by TCDD alone. An organic extract of fly ash from a municipal waste combustion plant was very potent. Considering its contents of polychlorinated dibenzo-p-dioxins/furans, expressed as TCDD equivalents, the EC50 obtained was close to that for TCDD.
机译：研制了一种体外研究鸡胚肝脏中7-乙氧基间苯二酚O-脱乙基酶（EROD）诱导的技术。在添加了DMSO的测试化合物的培养基中，将8 d大胚胎的肝脏在37℃的旋转小瓶中培养48小时。该生物测定法被证明对二恶英样化合物高度敏感，并证明了其对评估环境样品中此类化合物的毒性的有效性。确定了2,3,7,8-四氯二苯并-对-二恶英（TCDD），3,3'，4,4'，5-五氯联苯（PCB IUPAC编号126），3,3'， 4,4'-四氯联苯（PCB 77），2,3,3'，4,4'-五氯联苯（PCB 105）和苯并[k]荧蒽（BkF）。 TCDD以浓度依赖性方式诱导EROD，EC50为5.0 x 10〜（-12）M。培养的胚胎肝脏对TCDD极为敏感，每只肝脏约30 fg（2 x 10〜（- 13）M）足以显着诱导EROD。 PCB 126、77、105和BkF的EC50值分别为4.4 X 10〜（-11）M，9.2 X 10〜（-9）M，1.6 x 10〜（-8）M和6.2 x 10〜 （-7）M。三种不同的多氯化萘（PCN）制剂获得的最大诱导水平低于测试的其他化合物。当将工业PCN混合物Halowax 1014与TCDD并用时，诱导程度低于仅由TCDD引起的诱导程度。从城市垃圾焚烧厂提取的粉煤灰有机提取物非常有效。考虑到其多氯二苯并-对-二恶英/呋喃的含量（以TCDD当量表示），获得的EC50接近于TCDD。

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