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首页> 外文期刊>Euphytica >Three AFLP markers tightly linked to the genic male sterility ms3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker
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Three AFLP markers tightly linked to the genic male sterility ms3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker

机译：三个AFLP标记与辣椒（Capsicum annuum L.）的雄性不育ms 3 基因紧密相关并转化为CAPS标记

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摘要

Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms 3 gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms 3 locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.
机译：基因型雄性不育（GMS）长期以来一直用作辣椒（Capsicum annuum L.）杂交种子生产的工具。我们使用大分子隔离分析（BSA）和扩增的片段长度多态性（AFLP）技术开发了与隔离种群中GMS ms 3 基因相关的DNA标记。将分离的群体用512个引物组合进行BSA-AFLP。确定了三个AFLP标记（Eagg / Mccc 276 ，Eagc / Mctt 178 和Ecag / Mtgc 204 ）与ms < sub> 3 轨迹。其中，根据男性和女性之间的标记内部和侧面区域的测序分析，我们将AFLP标记Ecag / Mtgc 204 转换为名为GMS3-CAPS的裂解扩增多态序列（CAPS）标记。肥沃和不育的植物。该标记对于使用GMS系统进行胡椒育种非常有用。

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