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首页> 外文期刊>European Journal of Plant Pathology >Exploiting generic platform technologies for the detection and identification of plant pathogens
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Exploiting generic platform technologies for the detection and identification of plant pathogens

机译：开发用于检测和鉴定植物病原体的通用平台技术

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摘要

The detection and identification of plant pathogens currently relies upon a very diverse range of techniques and skills, from traditional culturing and taxonomic skills to modern molecular-based methods. The wide range of methods employed reflects the great diversity of plant pathogens and the hosts they infect. The well-documented decline in taxonomic expertise, along with the need to develop ever more rapid and sensitive diagnostic methods has provided an impetus to develop technologies that are both generic and able to complement traditional skills and techniques. Real-time polymerase chain reaction (PCR) is emerging as one such generic platform technology and one that is well suited to high-throughput detection of a limited number of known target pathogens. Real-time PCR is now exploited as a front line diagnostic screening tool in human health, animal health, homeland security, biosecurity as well as plant health. Progress with developing generic techniques for plant pathogen identification, particularly of unknown samples, has been less rapid. Diagnostic microarrays and direct nucleic acid sequencing (de novo sequencing) both have potential as generic methods for the identification of unknown plant pathogens but are unlikely to be suitable as high-throughput detection techniques. This paper will review the application of generic technologies in the routine laboratory as well as highlighting some new techniques and the trend towards multi-disciplinary studies.
机译：当前，植物病原体的检测和鉴定依赖于非常广泛的技术和技能范围，从传统的培养和生物分类技能到现代的基于分子的方法。所采用的方法广泛，反映出植物病原体及其感染宿主的多样性。有据可查的生物分类学专业知识的下降，以及对开发更加快速和灵敏的诊断方法的需求，为开发既通用又能够补充传统技能的技术提供了动力。实时聚合酶链反应（PCR）逐渐成为一种此类通用平台技术，并且非常适合对有限数量的已知目标病原体进行高通量检测。现在，实时PCR已被用作人类健康，动物健康，国土安全，生物安全以及植物健康的一线诊断筛查工具。开发用于植物病原体识别的通用技术，特别是未知样品的通用技术的进展较慢。诊断微阵列和直接核酸测序（从头测序）都有潜力作为识别未知植物病原体的通用方法，但不太可能适合用作高通量检测技术。本文将回顾通用技术在常规实验室中的应用，并重点介绍一些新技术和走向多学科研究的趋势。

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