Viability of Transgenic Rat Embryos after Freezing and Thawing

Masumi Hirabayashi; Ri-ichi Takahashi; Junko Sekiguchi

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Viability of Transgenic Rat Embryos after Freezing and Thawing

机译：冻融后转基因大鼠胚胎的生存力

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In-vivo viability of frozen-thawed embryos derived from transgenic rats, as well as the transmission and the expression of transgenes in the resultant newborn rats, was investigated. Three strains of transgenic rats, carrying human growth hormone gene connected downstream to the promoter region of the bovine α-lactalbumin gene (Αla/hGH), bovine β-casein gene (βCN/hGH) or bovine α-SI casein gene (αS1CN/hGH), were used. Two-cell stage embryos (non-transgenic Wistar female X heterozygous transgenic male) were Placed in 10/100 (v/v) dimethylsulfoxide (DMSO) solution and cooled from -7 to -30 deg. C at- 0.5 deg. C/min before being plunged into liquid nitrogen.

机译：研究了来自转基因大鼠的冻融胚胎的体内存活率，以及转基因动物在新生大鼠中的传递和表达。三株转基因大鼠，携带人类生长激素基因，其下游连接到牛α-乳清蛋白基因（Ala / hGH），牛β-酪蛋白基因（βCN/ hGH）或牛α-SI酪蛋白基因（αS1CN/ hGH）。将两细胞阶段的胚胎（非转基因Wistar雌性X杂合转基因雄性）置于10/100（v / v）二甲基亚砜（DMSO）溶液中，并从-7度冷却至-30度。 C在-0.5度。 C / min，然后放入液氮中。

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来源
《Experimental Animals》 |1997年第2期|p.111-115|共5页
作者
Masumi Hirabayashi; Ri-ichi Takahashi; Junko Sekiguchi;
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作者单位

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类动物学;
关键词
embryo freezing; gene expression; gene transmission; transgenic rat;

机译：胚胎冷冻;基因表达;基因传递转基因大鼠;

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1. Embryo quality before and after slow freezing: Viability, implantation and pregnancy rates in 627 single frozen-thawed embryo replacement cycles following failure of fresh transfer [J] . Capodanno Francesco, De Feo Gaetano, Gizzo Salvatore, SIAM journal on applied dynamical systems . 2017,第2期

机译：胚胎质量在缓慢冻结之前和之后：在新鲜转移失败后，627个单冰冻解冻胚胎置换循环中的可行性，植入和妊娠率
2. Sperm preparation after freezing improves motile sperm count, motility, and viability in frozen-thawed sperm compared with sperm preparation before freezing-thawing process [J] . A. Palomar Rios, A. Gascón, J. V. Martínez, Journal of assisted reproduction and genetics . 2018,第2期

机译：冷冻后的精子制备改善了冷冻解冻精子中的运动精液数，运动和活力，与冷冻后的精子制备相比
3. Blastocyst viability and generation of transgenic cattle following freezing of in vitro produced, DNA-injected embryos [J] . Y.M.Han, S.J.Kim, J.S.Park, Animal Reproduction Science . 2000,第1a2期

机译：冻存体外产生的DNA注射的胚胎后，胚泡活力和转基因牛的产生
4. Preservation of cell viability in fruit and vegetable tissues after freezing and thawing [C] . Federico Gómez Galindo, Petr Dejmek, Pui Yeu Phoon, International congress on engineering and food . 2011

机译：冻结和解冻后水果组织中的细胞活力保存
5. Experimental Study to Increase the Viability Rate of Cas9 Knock in Chicken Embryos and Examination of Differentially Expressed Genes in Early Chicken Embryos [D] . Byers, Mary Shannon. 2018

机译：试验研究，提高Cas9敲入鸡胚的活力率和早期鸡胚中的差异表达基因的检查
6. Sperm preparation after freezing improves motile sperm count motility and viability in frozen-thawed sperm compared with sperm preparation before freezing-thawing process [O] . A. Palomar Rios, A. Gascón, J. V. Martínez, 2018

机译：与冷冻-解冻前的精子制备相比冷冻后的精子制备可提高冻融精子的运动性精子数量运动性和活力。
7. O-197. Selection of viable embryos before freezing significantly improves the survival and pregnancy rates after thawing [O] . C. Wittemer, J. Ohl, K. Bettahar-Lebugle, 1997

机译：O-197。在冻结之前选择可行的胚胎显着提高了解冻后的存活率和妊娠速率
8. Viability and Function of Outdated Human Red Blood Cells after Biochemical Modification to Improve Oxygen Transport Function, Freezing, Thawing, Washing, Postthaw Storage at 4 C, Perfusion in Vitro through a Bubble Oxygenator, and [R] . Valeri, C. R., Vecchione, J. J., Pivacek, L. E., 1978

机译：生化修饰后过时的人红细胞的活力和功能，以改善氧传输功能，冷冻，解冻，洗涤，4℃的postthaw储存，通过气泡氧合器在体外灌注，和

Viability of Transgenic Rat Embryos after Freezing and Thawing

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