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首页> 外文期刊>Experimental Animals >Factors Affecting Full-Term Development of Rat Oocytes Microinjected with Fresh or Cryopreserved Round Spermatids
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Factors Affecting Full-Term Development of Rat Oocytes Microinjected with Fresh or Cryopreserved Round Spermatids

机译：影响新鲜或冷冻保存的圆形精子显微注射大鼠卵母细胞足月发育的因素

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Factors affecting full-term development of rat oocytes after round spermatid injection (ROSI) were examined using fresh and cryopreserved spermatids. Regardless of method (DC pulses or ionomycin) and timing (before or after ROSI) for oocyte activation, similar percentages of oocytes injected with cryopreserved spermatids developed to full-term (3.0-4.8%). In contrast, no offspring were obtained when fresh spermatids were injected into DC-treated oocytes (0%), but a higher proportion of oocytes first injected with fresh spermatids and then activated with ionomycin developed to full-term (6.1%). In further experiment, oocytes activated with ionomycin were injected with frozen-thawed spermatids, and then treated with cycloheximide and/or trichostatin-A. Neither chemical had a beneficial effect on full-term development of ROSI oocytes (3.7-7.9% vs 2.5% in control). In conclusion, regardless of timing of oocyte activation, activation treatment with ionomycin is required for full-term development of rat oocytes injected with fresh spermatids, and the higher potential of cryopreserved spermatids contributing to full-term development is notable when DC pulse is applied for oocyte activation.
机译：使用新鲜和冷冻保存的精子检查圆形精子注射（ROSI）后影响大鼠卵母细胞充分发育的因素。不管卵母细胞激活的方法（DC脉冲或离子霉素）和时间（ROSI之前或之后），注射冷冻保存的精子的卵母细胞的相似百分比都发展到足月（3.0-4.8％）。相反，当将新鲜的精子细胞注射到经DC处理的卵母细胞中时，没有获得后代（0％），但是首先注射新鲜的精子细胞然后被离子霉素激活的卵母细胞中有更高比例的卵子发育到足月（6.1％）。在进一步的实验中，将用离子霉素激活的卵母细胞注射冷冻融化的精子，然后用环己酰亚胺和/或曲古抑菌素-A处理。两种化学药品均未对ROSI卵母细胞的全面发育产生有益影响（3.7-7.9％，对照组为2.5％）。总之，无论卵母细胞活化的时间如何，注射新鲜精子的大鼠卵母细胞的充分发育都需要用离子霉素进行活化处理，而当施加直流脉冲时，低温保存的精子具有较高的潜能，可以充分发育。卵母细胞活化。

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