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首页> 外文期刊>Experimental Animals >Antibody response in non-human primates (Macaca fascicularis) immunized with short peptides derived from the envelope of the Dengue virus
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Antibody response in non-human primates (Macaca fascicularis) immunized with short peptides derived from the envelope of the Dengue virus

机译：用登革热病毒包膜衍生的短肽免疫的非人类灵长类动物（猕猴）的抗体反应

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Animal models for dengue virus infection have not been established so far. Non-human primates provide the closest model because they develop viremia with a time course that resembles human dengue virus infection. Such a response may be useful in testing candidate dengue vaccines which have so far not been successfully generated. The study aims to determine the antibody response in Macaca fascicularis immunized with short peptides derived from the envelope of the dengue virus. Briefly, four peptides that were bound by the monoclonal antibodies generated against the dengue 2 virus were selected from a phage display library. These peptides were synthesized and subcutaneously injected, individually and as a cocktail, in 1-year old monkeys, 5 times at 3-week intervals. Blood extraction (5 ml) was performed prior to each injection. For controls, monkeys were injected with the whole dengue virus and sterile distilled water, separately. Specific antibody response to the antigens was determined using ELISA. Microtiter plates were coated with the peptide cocktail to capture antibodies generated against the peptides. Peroxidase-antibody conjugates were used to visualize the captured anti-peptide antibodies via a color reaction which is measured using an ELISA-plate reader. Preliminary results showed that the monkeys injected with the peptide cocktail elicited antibodies to the peptides 3 weeks after the first injection, which doubled after the 2nd injection and was sustained throughout the rest of the duration. Monkeys injected with individual peptides developed antibodies after the second injection, but at a lower level in contrast with the peptide cocktail. Further studies are needed to determine if the monkey antibody response will be capable of neutralizing the dengue virus.
机译：迄今为止，尚未建立登革热病毒感染的动物模型。非人类的灵长类动物提供了最接近的模型，因为它们会随着类似于人类登革热病毒感染的时间进程而发展病毒血症。这样的应答可能在测试尚未成功产生的候选登革热疫苗中有用。这项研究的目的是确定用源自登革热病毒包膜的短肽免疫的猕猴的抗体反应。简而言之，从噬菌体展示文库中选择与针对登革热2病毒产生的单克隆抗体结合的四个肽。合成这些肽，并在1岁的猴子中以鸡尾酒的形式分别皮下注射，每3周间隔5次。每次注射前都要抽血（5毫升）。作为对照，分别给猴子注射整个登革热病毒和无菌蒸馏水。使用ELISA确定对抗原的特异性抗体应答。用肽混合物包被微量滴定板以捕获针对肽产生的抗体。过氧化物酶-抗体缀合物用于通过显色反应使捕获的抗肽抗体可视化，该显色反应使用ELISA板读数器测量。初步结果表明，注射了肽混合物的猴子在第一次注射后3周就产生了针对肽的抗体，在第二次注射后增加了一倍，并在其余时间中持续存在。注射了单个肽的猴子在第二次注射后会产生抗体，但与肽混合物相比却水平较低。需要进一步的研究以确定猴子抗体反应是否能够中和登革热病毒。

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