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Bordetella hinzii infection in a laboratory mouse and experimental study of the isolate

机译：小鼠中的豚鼠博德特氏菌感染及分离株的实验研究

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摘要

Bordetella hinzii was isolated from the trachea and lung of a laboratory mouse with a respiratory infection, and identified based on its phenotypic and genetic traits. The naturally infected mouse showed symptoms of sneezing, and histopathological examination revealed rhinitis, tracheitis, and bronchial pneumonia. The isolate was a gram-negative, oxidase- and catalase-positive, short rod-shaped organism that produced alkali from malonate. The results of biochemical identification by API 20NE and alkali production test from malonate, and partial sequence analysis of the 16S rRNA gene (1523 bp) were consistent with those reported previously for B. hinzii. The isolate induced sneezing in ICR mice by experimental infection. Histopathological examination revealed catarrhal rhinitis and bronchial pneumonia in the mice. The isolate was re-isolated from these lesions with pure culture. These results fulfilled Koch's postulates, and therefore B. hinzii was deemed to be a novel causative agent of respiratory disease in mice. This is the first report of natural infection by B. hinzii in mice and the first report confirming the pathogenicity of B. hinzii in a non-human mammal.
机译：从患有呼吸道感染的实验小鼠的气管和肺中分离出波氏杆菌，并根据其表型和遗传特征对其进行鉴定。自然感染的小鼠表现出打喷嚏的症状，组织病理学检查显示鼻炎，气管炎和支气管肺炎。分离物是革兰氏阴性，氧化酶和过氧化氢酶阳性的短杆状生物，可从丙二酸中产生碱。通过API 20NE进行的生化鉴定结果和丙二酸的碱生产测试结果以及16S rRNA基因（1523 bp）的部分序列分析与先前报道的B. hinzii一致。该分离株通过实验感染在ICR小鼠中引起打喷嚏。组织病理学检查显示小鼠中卡他性鼻炎和支气管肺炎。用纯培养物从这些病变中重新分离出分离物。这些结果满足了科赫的假设，因此，hinzii B.被认为是小鼠呼吸系统疾病的新型病原体。这是首次在小鼠中自然感染了B. hinzii的报告，并且首次证实了B. hinzii在非人类哺乳动物中的致病性。

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