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Improvement of medium for in vitro fertilization of inbred BALB/c mice

机译：自交BALB / c小鼠体外受精培养基的改良

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摘要

In vitro fertilization (IVF) of mice is used in reproduction research. However, the collection of embryos is often difficult because some strains show poor fertilization rates on IVF. The present study was performed to obtain IVF medium capable of supporting many strains. We compared the fertility rates in media consisting of modified human tubal fluid (mHTF) with and without addition of sodium lactate and containing various concentrations of calcium and glucose. The results of this study indicated that fertility improved with increased concentrations of calcium and glucose in the medium. In contrast, the fertilization rate decreased with the addition of sodium lactate during sperm capacitation. Based on these results, we examined sperm capacitated and inseminated medium, and have performed IVF with a stable high fertility rate of 70.3-91.5%. In addition, the IVF embryos showed ontogeny at a rate of more than 50%. Therefore, this medium appears to be effective for the preservation of strains and for production of the BALB/c strain.
机译：小鼠的体外受精（IVF）用于生殖研究。但是，胚胎收集通常很困难，因为某些菌株在IVF上受精率低。进行本研究以获得能够支持许多菌株的IVF培养基。我们比较了在添加和不添加乳酸钠以及含有各种浓度的钙和葡萄糖的改良人输卵管液（mHTF）组成的培养基中的生育率。这项研究的结果表明，随着培养基中钙和葡萄糖浓度的增加，生育能力得到改善。相反，在精子获能期间，受精率随乳酸钠的添加而降低。根据这些结果，我们检查了精子获能和受精的培养基，并以70.3-91.5％的稳定高生育率进行了IVF。此外，体外受精胚胎的发育发生率超过50％。因此，该培养基似乎对于菌株的保存和BALB / c菌株的产生是有效的。

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  • 来源
    《Experimental Animals》 |2008年第3supa期|p.99|共1页
  • 作者

    Tsutomu KAMISAKO; Tomoo ETO;

  • 作者单位

    Central Institute for Experimental Animals;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;
  • 关键词

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