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首页> 外文期刊>Experimental Animals >Detection of Cilia-Associated Respiratory Bacillus in Experimentally and Naturally Infected Mice and Rats by the Polymerase Chain Reaction
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Detection of Cilia-Associated Respiratory Bacillus in Experimentally and Naturally Infected Mice and Rats by the Polymerase Chain Reaction

机译：聚合酶链反应检测实验和自然感染小鼠和大鼠纤毛相关的呼吸道芽孢杆菌

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摘要

Cilia-associated respiratory (CAR) bacillus was detected by means of the reverse transcription (RT)-polymerase chain reaction (PCR), and the results were compared with those of indirect immunofluorescence test (IFAT) for the detection of the organism. In the experimental infections, 15 mice were in contact with mice previously inoculated with CAR bacillus. Three mice each were tested at days 3, 5, 7, 12 and 20 postexposure. On day 3 postexposure, CAR bacillus was detected in oral swab samples from all 3 mice by RT-PCR, but was not detected in any sampling sites from the mice by IFAT. Total numbers of positive samples from nasal, oral and tracheal swabs obtained through the test were 6/15, 14/15 and 8/15, respectively, by RT-PCR, and 2/15, 6/15 and 3/15, respectively by IFAT. For the detection of CAR bacillus in samples from 52 rats, 34 serum antibody negative rats by enzyme-linked immunosorbent assay were also negative by RT-PCR and IFAT except for one sample from the oral cavity, and all serum antibody positive rats were positive for the organism by RT-PCR but it could not be detected in five of them by IFAT. By means of RT-PCR, no differences in the positive rates depending on sampling sites were observed except in one rat. The RT-PCR was found to be a specific, highly sensitive and reliable procedure for detecting CAR bacillus in mice and rats. The oral cavity was the most suitable site for the diagnosis of the early stage of this infection by RT-PCR.
机译：通过逆转录（RT）-聚合酶链反应（PCR）检测纤毛相关呼吸道（CAR）细菌，并将结果与​​间接免疫荧光测试（IFAT）进行比较，以检测生物体。在实验性感染中，使15只小鼠与先前接种过CAR杆菌的小鼠接触。在暴露后第3、5、7、12和20天分别对三只小鼠进行测试。暴露后第3天，通过RT-PCR在所有3只小鼠的口腔拭子样本中检测到CAR杆菌，但通过IFAT在小鼠的任何采样部位均未检测到CAR杆菌。通过测试获得的鼻，口腔和气管拭子阳性样品总数通过RT-PCR分别为6 / 15、14 / 15和8/15，分别为2 / 15、6 / 15和3/15由IFAT。为了检测52只大鼠样品中的CAR杆菌，通过酶联免疫吸附法测定的34例血清抗体阴性大鼠也通过RT-PCR和IFAT呈阴性，除了来自口腔的一份样品外，所有血清抗体阳性的大鼠均为阳性。通过RT-PCR检测该生物，但是IFAT在其中五个中无法检测到。通过RT-PCR，除了一只大鼠外，没有观察到取决于采样部位的阳性率差异。发现RT-PCR是检测小鼠和大鼠中CAR芽孢杆菌的特异性，高度灵敏和可靠的方法。口腔是最适合通过RT-PCR诊断这种感染早期的部位。

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