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首页> 外文期刊>Experimental Brain Research >Peripheral nerve regeneration through alginate gel: analysis of early outgrowth and late increase in diameter of regenerating axons
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Peripheral nerve regeneration through alginate gel: analysis of early outgrowth and late increase in diameter of regenerating axons

机译：通过藻酸盐凝胶再生周围神经：早期轴突生长和再生轴突直径后期增加的分析

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摘要

Our previous study revealed that alginate gel cross-linked with covalent bonds promoted peripheral nerve regeneration in the cat and rat. The present study analyzed nerve regeneration through alginate gel in the early stages within 2 weeks and the late stages up to 21 months after implantation. Four days after surgery, regenerating axons grew without Schwann cell investment through the partially degraded alginate gel, being in direct contact with the alginate without a basal lamina covering. Numerous mast cells infiltrated into the alginate. One to 2 weeks after surgery, regenerating axons were surrounded by common Schwann cells to form small bundles, with some axons at the periphery being partly in direct contact with alginate. At the distal stump, numerous Schwann cells had migrated into the alginate 8–14 days after surgery. They had no basal laminae. The diameter of regenerated myelinated fibers was small (approximately 1 μm) at 8 weeks, but increased in diameter, having a distribution pattern similar to that of normal nerve 21 months after surgery. Much better nerve regeneration was found in alginate gel-, than collagen sponge-, and fibrin glue-implanted distal stump 12 months after surgery. These results indicate that alginate gel has good biocompatibility for regenerating axon outgrowth and Schwann cell migration, and that regenerated fibers can have a diameter as thick as that of normal fibers in the long term. Alginate gel is a promising material for use as an implant for peripheral nerve regeneration.
机译：我们以前的研究表明，藻酸盐凝胶与共价键交联可促进猫和大鼠的周围神经再生。本研究分析了藻酸盐凝胶在植入后2周内的早期和植入后直至21个月的后期神经再生。手术后四天，通过部分降解的藻酸盐凝胶，无需轴层覆盖，可直接与藻酸盐接触，从而在没有雪旺氏细胞投资的情况下再生轴突生长。大量肥大细胞渗入藻酸盐。手术后一到两周，再生的轴突被普通的雪旺氏细胞包围，形成小束，外围的一些轴突部分与藻酸盐直接接触。在远端残端，手术后8–14天大量的Schwann细胞已迁移到藻酸盐中。他们没有基底薄层。再生髓鞘纤维的直径在第8周较小（约1μm），但直径增大，在术后21个月具有与正常神经相似的分布方式。术后12个月，海藻酸盐凝胶（远比胶原海绵和纤维蛋白胶植入的远端残端）的神经再生效果好得多。这些结果表明，藻酸盐凝胶对于再生轴突生长和雪旺氏细胞迁移具有良好的生物相容性，并且从长远来看，再生纤维的直径可以与正常纤维一样大。海藻酸盐凝胶是一种有前途的材料，可用作周围神经再生的植入物。

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  • 来源
    《Experimental Brain Research》 |2002年第3期|356-368|共13页
  • 作者

    T. Hashimoto; Y. Suzuki; M. Kitada; K. Kataoka; S. Wu; K. Suzuki; K. Endo; Y. Nishimura; C. Ide;

  • 作者单位

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of MedicineDepartment of Anatomy and Neurobiology and Cell Biology Kyoto University Graduate School of Medicine;

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of Medicine;

    Department of Anatomy and Neurobiology and Cell Biology Kyoto University Graduate School of Medicine;

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of Medicine;

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of Medicine;

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of Medicine;

    Department of Physiology Kyoto University Graduate School of Medicine;

    Department of Plastic and Reconstructive Surgery Kyoto University Graduate School of Medicine;

    Department of Anatomy and Neurobiology and Cell Biology Kyoto University Graduate School of Medicine;

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  • 正文语种 eng
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  • 关键词

    Peripheral nerve regeneration; Alginate gel; Schwann cell migration; Axonal outgrowth; Schwann cell basal lamina; Immunohistochemistry; Electron microscopy; Electrophysiology;

    机译：周围神经再生;藻酸盐凝胶;Schwann细胞迁移;轴突生长;Schwann细胞基底层;免疫组织化学;电子显微镜;电生理学;

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