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首页> 外文期刊>Eye Science >Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush
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Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush

机译：大剂量甲基强的松龙对视神经粉碎后大鼠视网膜神经节细胞凋亡的影响

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摘要

Purpose: To investigate the effect of high dose methylprednisolone (MP) on retinal ganglion cells (RGC) apoptosis. Methods: One hundred and twenty six Wistar rats were divided into normal control, crush control and MP treatment groups. High dose MP and 0.9% sodium chloride were injected intravenously at 1 h after injury in the treatment group and crush control group, respectively. Apoptotic cells, Bcl-2 and Bax positive cells were located and calculated by terminal-deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemical method and retinal whole-mount combined with section technique at day 4, 7, 14 after crush, respectively. Results: The TUNEL-labeling cells, Bcl-2 and Bax positive cells were detected in the ganglion cell layer of retina. Few apoptotic cells, a few of Bcl-2 and Bax positive cells were observed in normal control groups. The numbers of positive cells increased gradually after crush. The number of apoptotic cells and Bax positive cells in treatment groups was less than that in crush group significantly (P < 0.05) , while the number of Bcl-2 positive cells in treatment group was more than that in crush group at day 4, 7 and 14 after crush significantly (P < 0.05) Conclusions: These results suggest that high dose MP can inhibit the apoptosis of RGC after optic nerve crush probably through up-regulation of Bcl-2 expression and down-regulation of Bax expression.
机译：目的：探讨大剂量甲基强的松龙（MP）对视网膜神经节细胞（RGC）凋亡的影响。方法：将126只Wistar大鼠分为正常对照组，挤压对照组和MP治疗组。治疗组和压溃对照组分别于伤后1 h静脉注射高剂量MP和0.9％氯化钠。在压榨后第4、7、14天，通过末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL），免疫组化方法和视网膜整装结合切片技术定位并计算凋亡细胞，Bcl-2和Bax阳性细胞。 ， 分别。结果：在视网膜神经节细胞层中检测到TUNEL标记细胞，Bcl-2和Bax阳性细胞。在正常对照组中观察到很少的凋亡细胞，少量的Bcl-2和Bax阳性细胞。压碎后，阳性细胞的数量逐渐增加。在第4、7天，治疗组的凋亡细胞和Bax阳性细胞数量明显少于挤压组（P <0.05），而治疗组的Bcl-2阳性细胞数量多于挤压组。结论：高剂量MP可以抑制视神经挤压后RGC的凋亡，其作用可能与Bcl-2表达的上调和Bax表达的下调有关。P <0.05和P <0.05。

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