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Effects of nerve growth factor on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium cells

机译:神经生长因子对培养的人胎儿视网膜色素上皮细胞增殖和DNA合成的影响

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Conclusion: The data suggested that NGF could stimulate the proliferation and DNA synthesis of cultured of hRPE cells in vitro in a dose-dependent manner. Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNA Synthesis of cultured human fetal retinal pigment epithelium(RPE)cells in vitro. Methods: Primary culture and subculture of human fetal retinal pigment epithelium cells Were established in vitro first. Cultured RPE cells were treated with NGF by various Concentrations 0μg/L, 50μg/L, 100μg/L, 200μg/L and 300μg/L(final concentration) For 48 hs.
机译:结论:数据表明NGF可以剂量依赖性刺激体外培养的hRPE细胞的增殖和DNA合成。目的:探讨神经生长因子(NGF)对体外培养的人胎视网膜色素上皮细胞(RPE)增殖和DNA合成的影响。方法:首先在体外建立人胎儿视网膜色素上皮细胞的原代培养和继代培养。用NGF分别以0μg/ L,50μg/ L,100μg/ L,200μg/ L和300μg/ L(终浓度)的不同浓度处理培养的RPE细胞48小时。

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