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首页> 外文期刊>Fish Physiology and Biochemistry >Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps
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Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps

机译：产卵前的水温影响雄性鲤鱼的精子呼吸和激活参数

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Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15°C (cold water (CW) group), whereas the second group was subjected to a temperature of 20°C (warm water (WW) group) before sperm sampling. Reactivation were achieved after incubation of firstly activated sperm in media with osmotic pressure adjusted up to 300 mOsm*kg−1 by increasing K+ concentration. Statistically significant reduction of spermatozoa concentration in CW samples versus WW (from 46.0 ± 12.5 (15°C) to 59.3 ± 7 109 (20°C) spermatozoa /ml) have been observed. The sperm of the CW group required a significantly longer incubation time (37 min) under isotonic conditions to achieve a maximum percentage of potent motility at repeated activation than the WW group (23 min). After activation of sperm motility, an increase of respiration rate up to maximum level has been found, this level remained the same under condition of recovering the potential to repeated activation. During the sperm movement respiration rate, in CW group (6.1 nmolO2/min/109spermatozoa) and WW (3.9 nmolO2/min/109spermatozoa), was significant higher compared to nonactivated sperm (2.4 nmolO2/min/109spermatozoa for CW and 1.1 nmolO2 /min/109spermatozoa for WW). And keeping males for 7 days at 15°C increase the respiration rate of sperm.
机译：在两个实验组鲤鱼雄性的精子中研究了浓度，运动能力，第二次激活（重新激活）过程中的运动以及内源性呼吸。第一组在15°C下保持7天（冷水（CW）组），而第二组在进行精子采样之前经受20°C的温度（温水（WW）组）。通过增加K + 浓度将首次激活的精子在渗透压调节至300 mOsm * kg -1 的培养基中孵育后，可以重新激活。观察到CW样品中精子浓度与WW相比有统计学意义的降低（从46.0±12.5（15°C）降至59.3±7 10 9 （20°C）精子/ ml）。与WW组（23分钟）相比，CW组的精子在等渗条件下需要显着更长的温育时间（37分钟），才能在重复激活时获得最大的运动力百分比。激活精子运动后，发现呼吸速率增加到最大水平，在恢复重复激活潜力的条件下，该水平保持不变。在精子运动呼吸速率中，连续波组（6.1 nmolO 2 / min / 10 9 精子）和WW（3.9 nmolO 2 / min） / 10 9 精子）显着高于未活化的精子（CW为2.4 nmolO 2 / min / 10 9 精子和1.1 nmolO < sub> 2 / min / 10 9 精子用于WW）。将雄性动物在15°C下放置7天会增加精子的呼吸速率。

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