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首页> 外文期刊>Fish Physiology and Biochemistry >Plasma FITC-dextran exchange between the primary and secondary circulatory systems in the Atlantic cod, Gadus Morhua
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Plasma FITC-dextran exchange between the primary and secondary circulatory systems in the Atlantic cod, Gadus Morhua

机译：大西洋鳕鱼Gadus Morhua一级和二级循环系统之间的血浆FITC-葡聚糖交换

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摘要

Fluorescein isothiocyanate dextran (FITC-dextran) exchange between the primary (PCS) and secondary (SCS) circulatory systems in the Atlantic cod, Gadus morhua (Linnaeus, 1752), were studied using 20-kDa (n = 4) and 500-kDa (n = 4) FITC-dextran. In order to give a qualitative perspective of the general connection between the PCS and SCS, distribution of plasma-borne tracers (FITC-dextran) in the PCS and SCS were examined. In this study, a total of eight cod were cannulated in the ventral aorta (PCS) and dorsal cutaneous vessel (SCS), for investigation of FITC-dextran disappearance in the PCS and its subsequent appearance in the SCS. FITC-dextran of both sizes was found to be in equilibrium between the PCS and SCS in less than 20 min. This indicates a profound connection between the PCS and SCS in the Atlantic cod, and rapid mixing of tracers between the PCS and SCS. The destination of the injected 500-kDa FITC-dextran was also examined, and it was observed that of the 500-kDa FITC-dextran lost from the primary and secondary vascular systems, 63.0 ± 9.2% could be recovered from the liver
机译：使用20 kDa（n = 4）和500 kDa研究了大西洋鳕鱼Gadus morhua（Linnaeus，1752）中主要（PCS）和次要（SCS）循环系统之间的异硫氰酸荧光素葡聚糖（FITC-葡聚糖）交换。 （n = 4）FITC-葡聚糖。为了定性分析PCS和SCS之间的一般连接，我们检查了PCS和SCS中血浆示踪剂（FITC-葡聚糖）的分布。在这项研究中，在腹主动脉（PCS）和背侧皮肤血管（SCS）中插管了总共八个鳕鱼，以研究FITC-右旋糖酐在PCS中的消失以及随后在SCS中的出现。发现两种尺寸的FITC-葡聚糖在不到20分钟的时间内即可在PCS和SCS之间达到平衡。这表明大西洋鳕鱼中PCS和SCS之间存在着深远的联系，PCS和SCS之间的示踪剂迅速混合。还检查了注射的500 kDa FITC-右旋糖酐的目的地，观察到从初级和次级血管系统中丢失的500 kDa FITC-右旋糖酐可从肝脏中回收63.0±9.2％

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