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首页> 外文期刊>Fish Physiology and Biochemistry >Transcript expression profiles of Takifugu rubripes spermatozoa and eggs by expressed sequence tag analysis
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Transcript expression profiles of Takifugu rubripes spermatozoa and eggs by expressed sequence tag analysis

机译：通过表达序列标签分析of红rub和卵的转录本表达谱

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摘要

Two cDNA libraries from Takifugu rubripes spermatozoa and eggs were constructed and a total of 620 expressed sequence tag (EST) clones were generated from the two libraries: 300 clones are from the spermatozoa library and 320 clones are from the eggs library. The most abundant cDNA clones in the two libraries were identified. A total of 207 ‘contigs’ (or single) EST clones were found to share significant sequence identity with known sequences in the GenBank database, representing at least 51 different genes. In order to understand the two types of germ cells further, the expression profiles of the identified clones in these cDNA libraries were analyzed. Furthermore, the presence of specific messenger RNAs in the spermatozoa and eggs has been demonstrated with BLAST analysis; the spermatozoa and egg library can supply unique and novel cDNA sequences in the Takifugu rubripes EST project. Another aim of this study is to identify cDNA clones that can be used as molecular markers for the analysis of the spermatogenesis and oogenesis in Takifugu rubripes. Six potential clones (S1-3 from spermatozoa and E1-3 from eggs) were selected to analyze their expression patterns by reverse transcription (RT)-PCR analyses. Half of these showed a specific expression in the expected tissue. Two of the clones were found by RT-PCR and in situ hybridization to be expressed specifically in the testis or ovary, and they maybe suitable molecular markers for the analysis of spermatogenesis and oogenesis.
机译：分别构建了来自红腹锦鸡和精子的两个cDNA文库，并从这两个文库生成了总共620个表达序列标签（EST）克隆：300个克隆来自精子文库，320个克隆来自卵文库。确定了两个文库中最丰富的cDNA克隆。发现总共207个“重叠群”（或单个）EST克隆与GenBank数据库中的已知序列具有重要的序列同一性，代表至少51个不同的基因。为了进一步了解这两种类型的生殖细胞，分析了这些cDNA文库中已鉴定克隆的表达谱。此外，通过BLAST分析已证明在精子和卵中存在特定的信使RNA。在Takifugu rubripes EST项目中，精子和卵文库可以提供独特而新颖的cDNA序列。这项研究的另一个目的是鉴定可以用作分子标记的cDNA克隆，用于分析红T鱼的精子发生和卵子发生。选择了六个潜在的克隆（来自精子的S1-3和来自卵的E1-3）通过逆转录（RT）-PCR分析来分析其表达模式。其中一半在预期组织中显示出特异性表达。通过RT-PCR和原位杂交发现其中两个克隆在睾丸或卵巢中特异性表达，它们可能是用于分析精子发生和卵子发生的合适分子标记。

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  • 来源
    《Fish Physiology and Biochemistry》 |2008年第3期|235-243|共9页
  • 作者

    Xue-Yan Shen; Jian-Zhou Cui; Qing-Li Gong; Yong-Jian Liu; Yoshitaka Nagahama;

  • 作者单位

    Division of Life Science and Technology Ocean University of China Qingdao 266003 P.R. China;

    Laboratory of Reproductive Biology National Institute for Basic Biology Okazaki 444-8585 Japan;

    Division of Life Science and Technology Ocean University of China Qingdao 266003 P.R. China;

    Division of Life Science and Technology Ocean University of China Qingdao 266003 P.R. China;

    Laboratory of Reproductive Biology National Institute for Basic Biology Okazaki 444-8585 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Spermatogenesis; Oogenesis; Germ cells; RT-PCR; ISH;

    机译：精子发生;卵子发生;生殖细胞;RT-PCR;ISH;

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