• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fisheries Science >Detection of cathepsin L in red cell membranes from fish blood
【24h】

Detection of cathepsin L in red cell membranes from fish blood

机译：鱼血中红细胞膜中组织蛋白酶L的检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cathepsin L, one of the cysteine proteases found in fish muscle, is considered to be the main cause of post-mortem autolysis of fish muscle. We have determined the presence of cathepsin L in the membranes of red blood cells of carp, amberjack, and red sea bream and measured its activity. Immunoblotting of an extract of the red blood cell membranes from these three fish species using human anti-cathepsin L antibody revealed the presence of cathepsin L of different molecular masses. The molecular masses of cathepsin L was estimated to be 120 and 85 kDa in the amberjack and 75 and 70 kDa in the carp. These proteins have higher molecular masses than the mature form of cathepsin L, suggesting that they are precursor forms. In contrast, the protein in the red sea bream was estimated to have a molecular mass of 30 kDa, suggesting that this cathepsin L is a mature form. The specific activity of cathepsin L was highest in the red blood cell membranes of the amberjack, followed by the carp and the red sea bream in descending order. Keywords Amberjack - Carp - Cathepsin L - Fish blood - Red cell membranes - Red sea bream
机译：组织蛋白酶L是鱼肌肉中发现的半胱氨酸蛋白酶之一，被认为是鱼肌肉事后自溶的主要原因。我们已经确定了组织蛋白酶L在鲤鱼，a鱼和红鲷鱼的红细胞膜中的存在，并测量了其活性。使用人抗组织蛋白酶L抗体对这三种鱼类的红细胞膜提取物进行了免疫印迹分析，结果显示存在不同分子量的组织蛋白酶L。组织蛋白酶L的分子量在a鱼中估计为120和85kDa，在鲤鱼中为75和70kDa。这些蛋白质比组织蛋白酶L的成熟形式具有更高的分子量，表明它们是前体形式。相反，红鲷鱼中的蛋白质估计分子量为30 kDa，表明该组织蛋白酶L是成熟形式。组织蛋白酶L的比活性在in鱼的红细胞膜中最高，其次是鲤鱼和红鲷，其降序排列。 A鱼-鲤鱼-组织蛋白酶L-鱼血-红细胞膜-红鲷鱼

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号