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首页> 外文期刊>Fisheries Science >Effect of long-term exposure to sulfides on taurine transporter gene expression in the gill of the deep-sea mussel Bathymodiolus platifrons, which harbors a methanotrophic symbiont
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Effect of long-term exposure to sulfides on taurine transporter gene expression in the gill of the deep-sea mussel Bathymodiolus platifrons, which harbors a methanotrophic symbiont

机译：长期接触硫化物对深海贻贝嗜盐菌（Bathymodiolus platifrons）the中甲烷营养共生体the中牛磺酸转运蛋白基因表达的影响

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摘要

Seawater around deep-sea hydrothermal vents and cold seeps contain high levels of hydrogen sulfide, which is toxic to most animals. Invertebrates inhabiting these environments have been reported to accumulate high levels of thiotaurine, a sulfur-containing amino acid. Thiotaurine is likely to play an important role in sulfide detoxification, but its functions in the detoxification process are still unknown. We cloned methane-seep mussel Bathymodiolus platifrons cDNA encoding the taurine transporter (TAUT), which transports thiotaurine and its precursors across the cell membrane. Phylogenetic analyses revealed that the predicted peptide formed a clade with the TAUTs of shallow-water mussel Mytilus galloprovincialis and the hydrothermal vent mussel Bathymodiolus septemdierum that harbors thioautotrophic bacteria. We then reared B. platifrons in the presence or absence of Na2S and quantified TAUT mRNA using a real-time PCR system. The amount of TAUT mRNA in the gills of B. platifrons increased with rearing in the presence of Na2S for 69 days, but no change was observed in the absence of sulfide. These results suggest that TAUT plays an important role in sulfide detoxification, even in species that do not harbor thioautotrophic bacteria. The TAUT mRNA level was variable in the mantle and low in the foot throughout the entire rearing period, regardless of the presence/absence of sulfide, suggesting that TAUT gene expression is regulated differentially in each tissue.
机译：深海热液喷口和冷泉周围的海水中含有大量的硫化氢，这对大多数动物都是有毒的。据报道，生活在这些环境中的无脊椎动物积累了高水平的硫牛磺酸（一种含硫氨基酸）。硫代牛磺酸可能在硫化物的解毒中起重要作用，但其在解毒过程中的功能仍然未知。我们克隆了编码甲烷的牛磺酸转运蛋白（TAUT），将甲烷深的贻贝Bathymodiolus platifrons cDNA编码，该蛋白可通过细胞膜转运硫代牛磺酸及其前体。系统发育分析表明，预测的肽与浅水贻贝Mytilus galloprovincialis的TAUT和含有硫代自养细菌的热液喷口贻贝Bathymodiolus septemdierum形成了进化枝。然后，我们在存在或不存在Na 2 S的情况下饲养了B. platifrons，并使用实时PCR系统对TAUT mRNA进行了定量。在Na 2 S存在下饲养69天后，platifrons the中TAUT mRNA的量增加，但是在不存在硫化物的情况下未观察到变化。这些结果表明，即使在不携带硫代自养细菌的物种中，TAUT在硫化物解毒中也起着重要作用。无论是否存在硫化物，TAUT mRNA的水平在整个饲养过程中在地幔中和脚的下部都是可变的，这表明TAUT基因的表达在每个组织中受到不同的调节。

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