...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fisheries Science >Structural characterization of plancitoxin I, a deoxyribonuclease II-like lethal factor from the crown-of-thorns starfish Acanthaster planci , by expression in Chinese hamster ovary cells
【24h】

Structural characterization of plancitoxin I, a deoxyribonuclease II-like lethal factor from the crown-of-thorns starfish Acanthaster planci , by expression in Chinese hamster ovary cells

机译：通过在中国仓鼠卵巢细胞中表达，对荆棘冠海星棘棘Acanthaster planci中的plancitoxin I（一种类似于脱氧核糖核酸酶II的致死因子）进行结构表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plancitoxin I, the major lethal factor from the spines of crown-of-thorns starfish Acanthaster planci, is a 37 kDa protein composed of two different subunits, and it has potent hepatotoxicity. It is homologous with mammalian deoxyribonuclease II (DNase II) and exhibits DNase activity responsible for the hepatotoxicity. To obtain information on the structure–activity relationship of plancitoxin I, various mutants were expressed in Chinese hamster ovary cells and examined for DNase activity. The results with deletion mutants revealed the requirement of the signal peptide for the expression of intact plancitoxin I and the inability of each subunit to hydrolyze DNA. Mutation at the N-glycosylation site (Asn-274) did not reduce DNase activity, supporting the absence of carbohydrate moieties in the molecule. The mutant H303A exhibited no DNase activity, suggesting the importance of His-303 for the enzymatic activity. No DNase activity was detected in C29A, C169A, C318A and C337A, indicating that four Cys residues are critical to the enzymatic activity. However, DNase activity was completely maintained in C263A and somewhat reduced in C277A and C357A. Based on the results with the Cys-specific mutants, plancitoxin I was assumed to contain three disulfide bridges (29–169, 277–357 and 318–337) and one free Cys-263.
机译：Plancitoxin I是荆棘冠海星棘棘棘棘的主要致死因子，是一种由两个不同亚基组成的37 kDa蛋白，具有强大的肝毒性。它与哺乳动物的脱氧核糖核酸酶II（DNase II）同源，并且表现出负责肝毒性的DNase活性。为了获得有关plancitoxin I的结构-活性关系的信息，各种突变体在中国仓鼠卵巢细胞中表达，并检测其DNase活性。缺失突变体的结果表明，信号肽需要完整的葡萄球菌毒素I表达，并且每个亚基均不能水解DNA。 N-糖基化位点（Asn-274）的突变不会降低DNase活性，从而支持分子中不存在碳水化合物部分。突变体H303A没有显示DNase活性，表明His-303对酶促活性的重要性。在C29A，C169A，C318A和C337A中未检测到DNase活性，表明四个Cys残基对酶促活性至关重要。但是，DNase活性在C263A中完全保持不变，而在C277A和C357A中有所降低。根据Cys特异性突变体的结果，普兰他辛I假定包含三个二硫键（29-169、277-357和318-337）和一个游离Cys-263。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号