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首页> 外文期刊>Food Additives & Contaminants >AFNOR validation of Premi~Rtest, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue
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AFNOR validation of Premi~Rtest, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue

机译：AFNOR验证Premi〜Rtest，这是一种基于微生物的筛查试管，用于检测动物肌肉组织中的抗菌素残留

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摘要

Premi~Rtest contains viable spores of a strain of Bacillus stearothermophilus which is sensitive to antimicrobial residues, such as beta-lactams, tetracyclines, macrolides and sulphonamides. The growth of the strain is inhibited by the presence of antimicrobial residues in muscle tissue samples. Premi~Rtest was validated according to AFNOR rules (French Association for Normalisation). The AFNOR validation was based on the comparison of reference methods (French Official method, I.e. four plate test (FPT) and the STAR protocol (five plate test)) with the alternative method (Premi~Rtest). A preliminary study was conducted in an expert laboratory (Community Reference Laboratory, CRL) on both spiked and incurred samples (field samples). Several method performance criteria (sensitivity, specificity, relative accuracy) were estimated and are discussed, in addition to detection capabilities. Adequate agreement was found between the alternative method and the reference methods. However, Premi~Rtest was more sensitive to beta-lactams and sulphonamides than the FPT. Subsequently, a collaborative study with 11 laboratories was organised by the CRL. Blank and spiked meat juice samples were sent to participants. The expert laboratory (CRL) statistically analysed the results. It was concluded that Premi~R Test could be used for the routine determination of antimicrobial residues in muscle of different animal origin with acceptable analytical performance. The detection capabilities of Premi~Rtest for beta-lactams (amoxicillin, ceftiofur), one macrolide (tylosin) and tetracycline were at the level of the respective maximum residue limits (MRL) in muscle samples or even lower.
机译：Pretest-Rtest含有嗜热脂肪芽孢杆菌菌株的活孢子，该菌株对诸如β-内酰胺类，四环素类，大环内酯类和磺酰胺类等抗菌残留物敏感。菌株的生长受到肌肉组织样品中抗菌素残留物的抑制。 Premi-Rtest根据AFNOR规则（法国标准化协会）进行了验证。 AFNOR验证基于参考方法（法国官方方法，即四板试验（FPT）和STAR方案（五板试验））与替代方法（Premi〜Rtest）的比较。在专家实验室（社区参考实验室，CRL）中对掺入样品和实际样品（田间样品）进行了初步研究。除检测功能外，还评估并讨论了几种方法的性能标准（敏感性，特异性，相对准确度）。在替代方法和参考方法之间找到了足够的一致性。但是，Premi-Rtest比β-内酰胺对β-内酰胺和磺酰胺更敏感。随后，CRL组织了与11个实验室的合作研究。将空白和加标的肉汁样品发送给参与者。专家实验室（CRL）对结果进行统计分析。结论是，Premi〜R试验可用于常规测定不同动物来源的肌肉中的抗菌素残留，并具有可接受的分析性能。 Premi〜Rtest对β-内酰胺类药物（阿莫西林，头孢噻呋），一种大环内酯类药物（泰乐菌素）和四环素的检测能力处于肌肉样品中各自最大残留限量（MRL）的水平，甚至更低。

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