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首页> 外文期刊>Food Chemistry >Selection of universal peptide biomarkers for the detection of the allergen hazelnut in food trough a comprehensive, high resolution mass spectrometric (HRMS) based approach
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Selection of universal peptide biomarkers for the detection of the allergen hazelnut in food trough a comprehensive, high resolution mass spectrometric (HRMS) based approach

机译：选择通用肽生物标记物，用于检测食槽中的过敏原榛子，这是一种基于全面，高分辨率质谱（HRMS）的方法

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摘要

The interest of using LC-MS/MS as a method for detection of allergens in food is growing. In such methods, peptides are used as biomarkers for the detection and quantification of the allergens. The selection of good biomarker peptides is of high importance to develop a specific, universal and sensitive method. Biomarkers should, for example, be robust to food processing. To evaluate robustness, test material incurred with hazelnut having undergone different food processing techniques was produced. Proteins of these materials were extracted, digested and further analyzed using HRMS. After peptide identification, selection was carried out using several criteria such as hazelnut specificity and amino acid composition. Further selection was done by comparing peptide MS intensities in the different food matrices. Only peptides showing processing robustness were retained. Eventually, eight peptides coming from three major hazelnut proteins were selected as the best biomarkers for hazelnut detection in processed foods.
机译：使用LC-MS / MS作为检测食物中过敏原的方法的兴趣正在增长。在这种方法中，肽被用作生物标志物，用于检测和定量变应原。良好的生物标志物肽的选择对于开发一种特定的，通用的和敏感的方法非常重要。例如，生物标志物应对食品加工具有鲁棒性。为了评估坚固性，生产了经历了不同食品加工技术的榛子试验材料。提取，消化和进一步使用HRMS分析这些物质的蛋白质。肽鉴定后，使用几种标准进行选择，例如榛子特异性和氨基酸组成。通过比较不同食品基质中肽MS的强度来进行进一步选择。仅保留显示加工鲁棒性的肽。最终，来自三种主要榛子蛋白的八种肽被选为检测加工食品中榛子的最佳生物标记。

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  • 来源
    《Food Chemistry》 |2020年第30期|125679.1-125679.10|共10页
  • 作者

  • 作者单位

    ILVO Flanders Res Inst Agr Fisheries & Food Technol & Food Sci Unit Brusselsesteenweg 370 BE-9090 Melle Belgium;

    CER Grp Rue Point Jour 8 B-6900 Marloie Belgium|Univ Namur Namur Res Inst Life Sci NARILIS Lab Biochem & Cell Biol URBC 61 Rue Bruxelles B-5000 Namur Belgium;

    Univ Namur Namur Res Inst Life Sci NARILIS Lab Biochem & Cell Biol URBC 61 Rue Bruxelles B-5000 Namur Belgium;

    CER Grp Rue Point Jour 8 B-6900 Marloie Belgium;

    Univ Ghent VIB Ctr Med Biotechnol Albert Baertsoenkaai 3 BE-9000 Ghent Belgium|Univ Ghent Dept Biomol Med Albert Baertsoenkaai 3 BE-9000 Ghent Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    High-resolution mass spectrometry; Peptide biomarker selection; Food processing; Allergen; Hazelnut;

    机译：高分辨率质谱;肽生物标志物选择;食品加工;过敏原;榛子;

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