Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

Baldassini Welder Angelo; Braga Camila Pereira; Loyola Chardulo Luis Artur; Ii Vasconcelos Silva Josineudson Augusto; Malheiros Jessica Moraes; de Albuquerque Lucia Galvao; Fernandes Talita Tanaka; Padilha Pedro de Magalhaes

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Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

机译：内洛尔品种（Bos indicus）肉嫩度不同等级的牛经鼻胸部肌肉组织的金属代谢组学研究的生物分析方法

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The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved.

机译：这项工作描述了用金属凝胶法对牛的内罗尔品种（Bos indicus）的动物具有不同等级的肉嫩度进行的金属组学研究，该蛋白基于二维凝胶电泳分离蛋白质，通过X射线荧光鉴定蛋白质斑点中的钙离子（SR-XRF）和通过电喷雾电离质谱分析蛋白质的特性。选择了四十（40）个标本，并将其分为两个实验组：带硬肉的动物（TO）和带嫩肉的动物（TE）。第三组（P）的皮埃蒙特犬种（Bos taurus）被用作肉嫩程度的比较模型。该程序有效并保留了金属蛋白质的结构，可以通过SR-XRF在14-97 kDa的给定分子量范围内检测蛋白质斑点中的钙。推断两种蛋白质（丙酮酸激酶和白蛋白）与不同组动物的表型差异有关。（C）2014 Elsevier Ltd.保留所有权利。

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来源
《Food Chemistry》 |2015年第15期|65-72|共8页
作者
Baldassini Welder Angelo; Braga Camila Pereira; Loyola Chardulo Luis Artur; Ii Vasconcelos Silva Josineudson Augusto; Malheiros Jessica Moraes; de Albuquerque Lucia Galvao; Fernandes Talita Tanaka; Padilha Pedro de Magalhaes;
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作者单位

Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Dept Anim Sci, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Dept Anim Sci, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo, Brazil;

Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo, Brazil;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类
关键词
2D-PAGE; Beef cattle; Calcium; Mass spectrometry; Zebu genotype;

机译：2D-PAGE;牛肉;钙;质谱;泽布基因型;

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专利

1. Expression of genes related to quality of Longissimus dorsi muscle meat in Nellore (Bos indicus) and Canchim (5/8 Bos taurusx3/8 Bos indicus) cattle [J] . Juliana Giusti, Eduardo Castan, Maeli Dal Pai, Meat Science . 2013,第2期

机译：与背最长肌肌肉肉品质相关的基因在内洛（Bos indicus）和Canchim（5/8 Bos taurusx3 / 8 Bos indicus）牛中的表达
2. Heat shock and structural proteins associated with meat tenderness in Nellore beef cattle, a Bos indicus breed [J] . Minos Esperandio Carvalho, Gustavo Gasparin, Mirele Daiana Poleti, Meat Science . 2014,第3期

机译：Bos indicus品种Nellore肉牛的热休克和与肉嫩有关的结构蛋白
3. Changes in protein abundance between tender and tough meat from bovine longissimus thoracis muscle assessed by isobaric Tag for Relative and Absolute Quantitation (iTRAQ) and 2-dimensional gel electrophoresis analysis. [J] . Bjarnadottir S. G, Hollung K, Hoy M, Journal of Animal Science . 2012,第6期

机译：通过等压定量绝对定量（iTRAQ）和二维凝胶电泳分析来评估牛胸肉肌肉中嫩肉和坚韧肉之间蛋白质丰度的变化。
4. Changes in protein composition related to tenderness in bovine longissimus thoracis muscle [C] . Hollung K., Faergestad E. M., Veiseth-Kent E. ICoMST 2011;International conference of meat science and technology . 2012

机译：牛胸最长肌肌肉压痛相关蛋白质组成的变化
5. Heritabilities and phenotypic and genetic correlations for bovine postrigor calpastatin activity and measures of meat tenderness and muscle growth. [D] . Shackelford, Steven Dean. 1992

机译：牛postrigor calpastatin活性的遗传力，表型和遗传相关性以及肉嫩度和肌肉生长的度量。
6. PSI-B-14 Late-Breaking: Evaluation of mRNA isoforms expression profile using two approaches to measure meat tenderness from Longissimus thoracis muscle in beef cattle [O] . Maria Malane M Muniz, Angela Cánovas, Larissa Fernanda Simielli Fonseca, 2019

机译：PSI-B-14晚期破裂：使用两种方法评估mRNA同种型表达谱的表达谱测量牛肉中的肌肉肌肉肌肉的肉嫩
7. Expression of genes related to quality of Longissimus dorsi muscle meat in Nellore (Bos indicus) and Canchim (5/8 Bos taurus×3/8 Bos indicus) cattle [O] . Giusti, Juliana [UNESP], Castan, Eduardo [UNESP], Dal Pai, Maeli [UNESP], 2013

机译：与牛背最长肌肉品质相关的基因在内罗（Nellore）和Can（Canchim，5/8 Bos taurus×3/8 Bos indicus）牛中的表达

Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

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