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首页> 外文期刊>Food Chemistry >Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle
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Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

机译:间接,非竞争性的安培免疫测定法,用于准确定量牛肌肉中钙压抑素(一种肉的嫩度标记)

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摘要

A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay.
机译:描述了一种通过安培检测定量肉样品中的牛肉嫩度标志物钙抑素的方法。使用新型牛重组部分钙蛋白酶抑制剂蛋白作为标准抗原,可实现0.2 ng / mL的低检测限。在检测钙蛋白酶抑制素之前,通过热处理和样品稀释可以最大程度地降低复杂基质的影响。直接样品测量和标准添加方法之间的相对误差为5.89%,证实了开发的安培免疫分析的准确性。

著录项

  • 来源
    《Food Chemistry》 |2012年第2期|p.598-603|共6页
  • 作者单位

    Department of Biotechnology, Lund University, 22100 Lund, Sweden;

    Department of Biotechnology, Lund University, 22100 Lund, Sweden,Department of Food Technology, Engineering and Nutrition, Lund University, 22100 Lund, Sweden;

    Department of Biochemistry and Structural Biology/Analytical Chemistry, Lund University, 22100 Lund, Sweden;

    Department of Biotechnology, Lund University, 22100 Lund, Sweden,Institute of Food, Nutrition and Health, Swiss Federal Institute of Technology Zurich, 8092 Zurich, Switzerland;

    Department of Biotechnology, Lund University, 22100 Lund, Sweden,Department of Biology, University of Erciyes, 38039 Kayseri, Turkey;

    Department of Biochemistry and Structural Biology/Analytical Chemistry, Lund University, 22100 Lund, Sweden;

    Division of Nutritional Sciences, University of Nottingham, LE12 5RD, Nottingham, UK;

    Department of Biotechnology, Lund University, 22100 Lund, Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    matrix effect; raw meat; calpastatin; amperometry; immunoassay;

    机译:基质效应生肉钙抑素安培法;免疫测定;

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