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首页> 外文期刊>Food Chemistry >DDDetermination of resveratrol in grains, hulls and leaves of common and tartary buckwheat by HPLC with electrochemical detection at carbon paste electrode
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DDDetermination of resveratrol in grains, hulls and leaves of common and tartary buckwheat by HPLC with electrochemical detection at carbon paste electrode

机译：高效液相色谱-碳糊电极电化学检测法测定普通荞麦和苦荞谷物，壳和叶中白藜芦醇的含量

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摘要

A reverse-phase high-performance liquid chromatographic method for the determination of rrans-resve-ratrol with spectrophotometric detection (306 nm) and amperometric detection at carbon paste electrode (E = + 1,2 V) was developed and tested on real samples of grains, hulls and leaves of six varieties of common buckwheat (Fagopyrum esculentum Moench) and two varieties of tartary buckwheat (Fagopy-rum tataricum (L.) Caertn.). Optimal conditions for the determination of trans-resveratrol were as follows: column Kromasil C-18 (7 μrn), 125 x 4 mm; mobile phase acetonitrile: diluted BR buffer pH 7 (50:50, 30:70 for grains and hulls and 20:80 for leaves); flow rate 1 ml min~(-1). Under these conditions, the limit of detection of trans-resveratrol (LD) was 3.5 × 10~(-8) mol 1~(-1) (R~2 = 0.9986) for electrochemical detection and 3.2 × 10~(-8) mol l~(-1) (R~2 = 0.9993) for spectrophotometric detection.
机译：开发了一种反相高效液相色谱法，用于分光光度法检测（306 nm）和碳糊电极上的安培检测（E = + 1,2 V），用于测定Rrans-resve-ratrol。六种普通荞麦（Fagopyrum esculentum Moench）和两种t苦荞（Fagopy-rum tataricum（L.）Caertn。）的谷物，壳和叶。测定反式白藜芦醇的最佳条件如下：Kromasil C-18柱（7μm），125 x 4 mm；流动相乙腈：稀释的BR缓冲液pH 7（谷物和船体的比例为50：50，30：70，叶片为20:80）；流速1 ml min〜（-1）。在这种条件下，用于电化学检测的反白藜芦醇（LD）的检出限为3.5×10〜（-8）mol 1〜（-1）（R〜2 = 0.9986）和3.2×10〜（-8）分光光度法检测：mol l〜（-1）（R〜2 = 0.9993）。

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  • 来源
    《Food Chemistry》 |2011年第1期|p.374-378|共5页
  • 作者

    Lenka Nemcova; Jiff Zima; Jifi Barek; Dagmar Janovska;

  • 作者单位

    Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry,Albertov 6, 728 43 Prague, Czech Republic;

    Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry,Albertov 6, 728 43 Prague, Czech Republic;

    Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry,Albertov 6, 728 43 Prague, Czech Republic;

    Crop Research Institute, Department ofCene Bank, Drnovska 507, 16! 06 Prague, Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    trans-resveratrol; buckwheat; hplc; electrochemical detection; carbon paste electrode; spectrophotometric detection;

    机译：反式白藜芦醇荞麦hplc电化学检测碳糊电极分光光度法;

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