Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

David Lee; Maurizio La Mura; Andrew Greenland; Ian Mackay

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Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

机译：使用信息性零值（QUIZ）进行定量：无需进行认证的参考物质即可进行GMO检测和定量的应用

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The amount of genetically modified RoundUp Ready™ soya in admixture samples was determined using a method, quantitation using informative zeros that employs most probable number statistics to estimate molecule numbers. By measuring the numbers of lectin, acting as a reference marker, and GMO-specific targets within these samples, we were able to determine their GMO contents. Estimates derived from QUIZ were comparable with results obtained using conventional real-time Taqman~® PCR. Results of GMO content determination using QUIZ is based on a 'per genome' units and estimations can be obtained using very small amounts of DNA. Certified reference materials, constructed using weight measurements, were not required for quantification.

机译：混合样品中基因修饰的RoundUp Ready™大豆的量是通过使用信息性零点进行定量的方法确定的，该零点采用最可能的数量统计数据来估算分子数量。通过测量这些样本中凝集素的数量，用作参考标记以及GMO特异性靶标，我们能够确定其GMO含量。从QUIZ得出的估计值与使用常规实时Taqman®PCR获得的结果相当。使用QUIZ进行GMO含量测定的结果基于“每个基因组”单位，可以使用非常少量的DNA进行估算。使用重量测量构建的认证参考材料不需要进行定量。

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来源
《Food Chemistry》 |2010年第4期|974-978|共5页
作者
David Lee; Maurizio La Mura; Andrew Greenland; Ian Mackay;
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作者单位

National Institute of Agricultural Botany, Huntingdon Road. Cambridge CAMBS CB3 0LE, United Kingdom;

National Institute of Agricultural Botany, Huntingdon Road. Cambridge CAMBS CB3 0LE, United Kingdom Dipartimento di Scienze del Suolo, delta Pianta, dell' Ambiente e delle Produzioni animali, University of Naples, Federico II, Via Universita 100, 80055 Portici, Italy;

National Institute of Agricultural Botany, Huntingdon Road. Cambridge CAMBS CB3 0LE, United Kingdom;

National Institute of Agricultural Botany, Huntingdon Road. Cambridge CAMBS CB3 0LE, United Kingdom;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
原文格式 PDF
正文语种 eng
中图分类
关键词
DNA quantitation; GMO content determination; single molecule amplification and detection; roundUp ready™ soya; transgene; lectin;

机译：DNA定量;转基因生物含量测定;单分子扩增和检测;roundUp ready™大豆;转基因凝集素;

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Quantitation using informative zeros (QUIZ): Application for GMO detection and quantification without recourse to certified reference material

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