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首页> 外文期刊>Food Chemistry >Optimised Ultrasonic-assisted Extraction Of Flavonoids From Folium Eucommiae And Evaluation Of Antioxidant Activity In Multi-test Systems In Vitro
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Optimised Ultrasonic-assisted Extraction Of Flavonoids From Folium Eucommiae And Evaluation Of Antioxidant Activity In Multi-test Systems In Vitro

机译：杜仲叶中黄酮的超声辅助提取工艺优化及体外多试验体系抗氧化活性的评价

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摘要

The central composite design combined with response surface methodology was used to optimise ultrasonic-assisted extraction for flavonoids-enriched extract from Folium eucommiae. The results indicate that the highest extraction ratio of flavonoids by ultrasonic-assisted extraction could arrive 17.2% using 40% ethanol as solvent and 1:60 solid to lipid ratio for 70 min extraction, which was more efficient than that by heating, microwaves-assisted and enzyme-assisted extractions. The crude extract was then purified by macroporous adsorption resin, the flavonoids content in the purified extract increased to 78.6%. The flavonoids-enriched extract exhibited quite strong reducing property as well as the total antioxidant capacity, and excellent inhibitions of hydroxyl radical, superoxide radical, the haemolysis of rat erythro-cytes and the generation of MDA of rat organ tissue. Meanwhile, it was found that the bioactivities appeared to be dose-dependent of flavonoids.
机译：采用中央复合设计与响应面方法相结合的方法，对杜仲叶中富含类黄酮的提取物进行了超声波辅助提取的优化。结果表明，以40％乙醇为溶剂，固液比为1:60的超声提取70min，超声辅助提取黄酮的最高提取率可达17.2％，比加热，微波辅助提取效率更高。和酶辅助提取。然后通过大孔吸附树脂纯化粗提取物，纯化的提取物中的类黄酮含量增加至78.6％。富含类黄酮的提取物表现出相当强的还原性以及总的抗氧化能力，并且对羟自由基，超氧化物自由基，大鼠红细胞的溶血和大鼠器官组织的MDA生成具有出色的抑制作用。同时，发现黄酮类化合物的生物活性似乎与剂量有关。

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