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Reduction of Escherichia coli, as a surrogate for Salmonella spp., on the surface of grapefruit during various packingline processes

机译:在各种包装生产线过程中减少大肠杆菌(作为沙门氏菌属的替代品)在葡萄柚表面上的表达

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The US Produce Safety Rule allows for use of water that does not meet its microbial standards if corrective measures are employed. This research was initiated to determine the suitability of nonpathogenic Escherichia coli as a surrogate for Salmonella during citrus washing, and to evaluate the removal of E. colt from grapefruit on two pilot packinglines (CREC and IRREC) as corrective measures. Whole grapefruit were inoculated with either E. coll. or Salmonella and dried, and exposed to a variety of treatments on a lab-scale brush wash system. Individual processes were evaluated on the pilot packinglines with E. coll. only. In all lab-scale brush wash system treatments, bacterial population reductions between E. coll. and Salmonella were not significantly different (P = 0.05). On pilot packinglines, E. colt populations were reduced by 3.59 to 5.11 log CFU/grapefruit at the CREC packingline, and by 3.30 to 5.13 log CFU/grapefruit at the IRREC packingline. Treatment of fruit through complete packingline processing at both locations reduced E. coli populations to levels below the detection limit ( 1 log CFU/grapefruit). The studies indicate E. coli is an appropriate surrogate for Salmonella under tested conditions, and that standard citrus packingline processes can be used as a corrective measure.
机译:如果采取纠正措施,《美国农产品安全规则》允许使用不符合其微生物标准的水。这项研究的开始是为了确定非病原性大肠杆菌在柑橘洗涤过程中作为沙门氏菌替代品的适用性,并评估在两个试验包装生产线(CREC和IRREC)上从葡萄柚中去除大肠杆菌的作为纠正措施。整个葡萄柚都接种了大肠杆菌。沙门氏菌或沙门氏菌并干燥,然后在实验室规模的刷洗系统上进行多种处理。在E. coll的中试装箱线上评估了各个过程。只要。在所有实验室规模的刷洗系统处理中,大肠杆菌之间的细菌种群减少。与沙门氏菌无显着差异(P <= 0.05)。在试点包装生产线上,柯尔特大肠杆菌的种群在CREC包装生产线上减少了3.59对> 5.11 log CFU /葡萄柚,在IRREC包装生产线上减少了3.30对数CFU /葡萄柚。通过在两个位置通过完整的包装生产线处理来处理水果,可将大肠埃希菌数量降低到检测限以下(<1 log CFU /葡萄柚)。研究表明,在测试条件下,大肠杆菌是沙门氏菌的适当替代品,并且标准柑橘包装生产线工艺可用作纠正措施。

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