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首页> 外文期刊>Food microbiology >Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains
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Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains

机译：暴露于冷和冷渗透胁迫下对单核细胞增生李斯特菌菌株毒力相关特征的影响

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The objective of this work was to investigate the effect of stress conditions frequently encountered in food-associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 ℃, cold stress) and optimal (37 ℃) temperatures and in high NaCl concentrations (6% NaCl, 11 ℃; cold-osmotic stress) and tested for their ability to invade the human intestinal epithelial Caco-2 cells. Results demonstrate that the correlation between exposure to cold stress and increased invasion phenotype is strain-dependent as strains investigated exhibited different behaviours, i.e. exposure to cold stress conditions resulted in a significant increase of invasion levels in five out of the eight strains tested, when compared to growth under optimal conditions. On the other hand, when these cold-adapted cells were subsequently submitted to high salt concentrations and low temperature, their enhanced ability to invade Caco-2 was lost. Surprisingly, saturated fatty acids (SFA) and branched chain fatty acids (BCFA) decreased when L. monocytogenes were exposed to stress conditions as opposed to what has been observed in other studies, therefore highlighting that further studies will need to deepen in the understanding of the lipid metabolism of these strains. The effect of stress conditions on the survival of three selected L. monocytogenes strains through an in vitro gastrointestinal (GI) tract digestion model was further investigated. The exposure to cold-osmotic stress increased the survival of one strain through the GI tract.
机译：这项工作的目的是调查食品相关环境中经常遇到的压力条件对八种单核细胞增生李斯特菌菌株毒力相关特征的影响。菌株在低温（11℃，冷胁迫）和最佳温度（37℃）和高氯化钠浓度（6％氯化钠，11℃;冷渗透胁迫）下生长，并测试其侵袭人类肠道上皮Caco-的能力。 2个细胞。结果表明，暴露于冷胁迫下与入侵表型增加之间的相关性取决于菌株，因为所研究的菌株表现出不同的行为，即，与之相比，暴露于寒冷胁迫条件下，所测试的八种菌株中有五种的入侵水平显着增加。在最佳条件下生长。另一方面，当这些冷适应的细胞随后置于高盐浓度和低温下时，它们增强的入侵Caco-2的能力就会丧失。出乎意料的是，当单核细胞增生李斯特菌暴露于应激条件下时，饱和脂肪酸（SFA）和支链脂肪酸（BCFA）下降，这与其他研究中所观察到的相反，因此强调了进一步的研究将需要加深对这些菌株的脂质代谢。通过体外胃肠道（GI）消化模型，进一步研究了应激条件对三种选定的单核细胞增生李斯特菌菌株存活的影响。暴露于冷渗透胁迫下增加了一种菌株在胃肠道中的存活。

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