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首页> 外文期刊>Food microbiology >Proteomic analysis of the food spoiler Pseudomonas fluorescens ITEM 17298 reveals the antibiofilm activity of the pepsin-digested bovine lactoferrin
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Proteomic analysis of the food spoiler Pseudomonas fluorescens ITEM 17298 reveals the antibiofilm activity of the pepsin-digested bovine lactoferrin

机译：食物扰流板的蛋白质组学分析荧光荧光素项17298揭示了胃蛋白酶消化牛乳蛋白的抗生素活性

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摘要

Pseudomonas fluorescens is implicated in food spoilage especially under cold storage. Due to its ability to form biofilm P. fluorescens resists to common disinfection strategies increasing its persistance especially across fresh food chain. Biofilm formation is promoted by several environmental stimuli, but gene expression and protein changes involved in this lifestyle are poorly investigated in this species.In this work a comparative proteomic analysis was performed to investigate metabolic pathways of underlying biofilm formation of the blue cheese pigmenting P. fluorescens ITEM 17298 after incubation at 15 and 30 degrees C; the same methodology was also applied to reveal the effects of the bovine lactoferrin hydrolysate (HLF) used as antibiofilm agent.At 15 degrees C biofilm biomass and motility increased, putatively sustained by the induction of regulators (PleD, AlgB, CsrA/RsmA) involved in these phenotypic traits. In addition, for the first time, TycC and GbrS, correlated to indigoidine synthesis (blue pigment), were detected and identified. An increase of virulence factors amounts (leukotoxin and PROKKA_04561) were instead found at 30 degrees C. HLF caused a significant reduction in biofilm biomass; indeed, at 15 degrees C HLF repressed PleD, TycC and GbrS and induced the negative regulators of alginate biosynthesis; at both temperatures induced the cyclic-di-GMP-binding biofilm dispersal mediator (PROKKA_02061).In conclusion, in this work protein determinats of biofilm formation were revelead in ITEM 17298 under the low temperature; the synthesis of these latter were inhibited by HLF confirming its possible exploitation as antibiofilm agent for biotechnological applications in cold stored foods.
机译：假单胞菌荧光型在蓄冷下涉及食物腐败。由于其能够形成生物膜P.荧光致力于常见的消毒策略，其持久性尤其是在新鲜食物链上。生物膜形成由几种环境刺激促进，但在该物种中涉及的基因表达和涉及该生活方式的蛋白质变化。在这项工作中，进行了对比较蛋白质组学分析，以研究蓝芝士色素的底层生物膜形成的代谢途径。在15℃和30摄氏度孵育后荧光物品17298;还应用了相同的方法，揭示了用作抗噻唑米蛋白剂的牛乳铁蛋白水解产物（HLF）的作用。15摄氏度的生物膜生物质和动力增加，通过诱导调节剂（Pled，AlGB，CSRA / RSMA）所涉及的诱导持续在这些表型特征中。此外，检测和鉴定与Incigoidine合成（蓝色颜料）相关的TYCC和GBRS。在30摄氏度下发现了毒力因子量（白曲毒素和Prokka_04561）的增加.HLF导致生物膜生物质显着降低;实际上，在15摄氏度的HLF抑制镀层，TYCC和GBR，并诱导了藻酸盐生物合成的负调节剂;在两种温度下，诱导环状二基 - GMP结合的生物膜分散介质（Prokka_02061）。在该工作中，在该工作中，生物膜形成的蛋白质测定在低温下在第17298项中重新定义;通过HLF抑制了这些后者的合成证实其可能的剥削作为冷储存食品中的生物技术应用的抗生素剂。

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  • 来源
    《Food microbiology》 |2019年第9期|177-193|共17页
  • 作者

    Quintieri Laura; Zuehlke Daniela; Fanelli Francesca; Caputo Leonardo; Liuzzi Vania Cosma; Logrieco Antonio Francesco; Hirschfeld Claudia; Becher Dorte; Riedel Katharina;

  • 作者单位

    Natl Res Council Italy Inst Sci Food Prod CNR ISPA Via G Amendola 122-0 I-70126 Bari Italy;

    Ernst Moritz Arndt Univ Greifswald Inst Microbiol D-17487 Greifswald Germany;

    Natl Res Council Italy Inst Sci Food Prod CNR ISPA Via G Amendola 122-0 I-70126 Bari Italy;

    Natl Res Council Italy Inst Sci Food Prod CNR ISPA Via G Amendola 122-0 I-70126 Bari Italy;

    Natl Res Council Italy Inst Sci Food Prod CNR ISPA Via G Amendola 122-0 I-70126 Bari Italy;

    Natl Res Council Italy Inst Sci Food Prod CNR ISPA Via G Amendola 122-0 I-70126 Bari Italy;

    Ernst Moritz Arndt Univ Greifswald Inst Microbiol D-17487 Greifswald Germany;

    Ernst Moritz Arndt Univ Greifswald Inst Microbiol D-17487 Greifswald Germany;

    Ernst Moritz Arndt Univ Greifswald Inst Microbiol D-17487 Greifswald Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Food spoilers; Pigments; Temperature adaptation; Antimicrobial peptides; Genomics; GeLC-MS/MS;

    机译：食物扰流板;颜料;温度适应;抗微生物肽;基因组学;GELC-MS / MS;

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