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首页> 外文期刊>Food microbiology >Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei
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Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei

机译：QPCR在乳酸杆菌中的生物胺降解中的多球氧化酶基因（MCO）的应用

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摘要

Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we designed two sets of primers for the detection and quantification of the multicopper oxidase gene (MCO), which encodes an enzyme involved in BAs degradation, and endogenous (glyceraldehyde-3-phosphate dehydrogenase) gene (GAPDH) in Lactobacillus casei group by real-time PCR (qPCR). We tested 15 Lactobacillus strains in the screening assays (thus, MCO gene possessing assay (PCR) and monitoring of BAs degradation by HPLC-UV), in which Lactobacillus casei CCDM 198 exhibited the best degradation abilities. For this strain, we monitored the expression of the target gene (MCO) in time (qPCR), the effect of redox treatments (cysteine, ascorbic acid) on the expression of the gene, and the ability to degrade BAs not only in a modified MRS medium (MRS/2) but also in a real food sample (milk). Moreover, decarboxylase activity (ability to form BAs) of this strain was excluded. According to the results, CCDM 198 significantly (P ＜ 0.05) reduced BAs (putrescine, histamine, tyramine, cadaverine), up to 25% decline in 48 h. The highest level of relative expression of MCO (5.21 ± 0.14) was achieved in MRS/2 media with cysteine.
机译：通过微生物的食品中不希望的生物胺（BAS）的降解被认为是消除毒性最有效的方法之一。在这项研究中，我们设计了两组引物，用于检测和定量多氧化物酶基因（MCO），其编码参与基础降解的酶，内源（甘油醛-3-磷酸脱氢酶）基因（GAPDH）在乳酸杆菌中通过实时PCR（QPCR）进行组。我们在筛选测定中测试了15个乳杆菌菌株（因此，具有测定（PCR）的MCO基因和通过HPLC-UV监测BAS降解），其中Lactobacillus Cassi CCDM 198表现出最佳的降解能力。对于这种菌株，我们监测靶基因（MCO）的表达（QPCR），氧化还原处理（半胱氨酸，抗坏血酸）对基因表达的影响，以及降解碱化的能力不仅在改性中MRS媒介（MRS / 2），但也在真正的食物样本（牛奶）中。此外，排除了该菌株的脱羧酶活性（形成BAS的能力）。根据结果​​，CCDM 198显着（P <0.05）降低（Putrescine，组胺，酪胺，尸鼠），48小时达25％下降。 MCO（5.21±0.14）的最高水平的MCO（5.21±0.14）在半胱氨酸中达到MRS / 2培养基。

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  • 来源
    《Food microbiology》 |2020年第10期|103550.1-103550.8|共8页
  • 作者

    Hana Pistekova; Petra Jancova; Lucie Bercikova; Frantisek Bunka; Iveta Sokolova; Tomas Sopik; Kristyna Marsalkova; Olga Maria Reis Pacheco de Amaral; Leona Bunkova;

  • 作者单位

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Food Technology Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Food Technology Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

    Department of Technologies and Applied Sciences Escola Superior Agaria Instituto Politecnico de Beja Rua Pedro Soares S/N Apartado 6155 7800-295 Beja Portugal;

    Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlin Vavreckova 275 76001 Zlin Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biogenic amines degradation; Histamine; qPCR; Primers; Lactobacillus casei;

    机译：生物胺降解;组胺;QPCR;引物;乳杆菌酪蛋白;

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