• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Efficacy of nisin derivatives with improved biochemical characteristics, alone and in combination with endolysin PlyP100 to control Listeria monocytogenes in laboratory-scale Queso Fresco
【24h】

Efficacy of nisin derivatives with improved biochemical characteristics, alone and in combination with endolysin PlyP100 to control Listeria monocytogenes in laboratory-scale Queso Fresco

机译：Nisin衍生物具有改善的生化特征，单独和与眼糖苷蛋白普利普蛋白的疗效组合在实验室规模Queso Fresco中对照中的单核细胞增生中的疗效

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nisin is an antimicrobial peptide that is commonly used as a food preservative and capable of inhibiting the pathogen Listeria monocytogenes. However, nisin is ineffective in controlling L. monocytogenes in Queso Fresco (QF). To address the challenge, in this work, we used synthetic biology strategies to create a series of nisin A derivatives by substituting residues 27, 30, 31 and 32 with positively charged amino acids (H, K and R). Our results showed that nisin derivatives exhibited reduced antilisterial activity in vitro compared to nisin A; however, they were all more stable under QF-like experimental conditions (pH 7 + 22% milk fat), notably H27/31K. Compared to nisin A, the derivatives H31K and V32K exhibited slight antilisterial improvement in QF and H27/ 31K was able to reduce the initial population of L. monocytogenes by up to 1.5 Log CFU/g. L. monocytogenes isolates exhibited similar susceptibility to nisin A or H27/31K after 7 or 14 days of nisin exposure in QF. Notably, when combined with endolysin PlyP100, the application of H27/31K resulted in non-enumerable levels of L. monocytogenes after 14 days of cold storage. Our results highlight the potential of bioengineered nisin derivatives for stabilized and enhanced control of L. monocytogenes in QF.
机译：Nisin是一种抗微生物肽，通常用作食品防腐剂，并且能够抑制病原体李斯特菌单核细胞增生。然而，Nisin在控制Queso Fresco（QF）中的L.单核细胞增生中是无效的。为了解决挑战，在这项工作中，我们使用合成生物学策略来通过用带正电荷的氨基酸（H，K和R）代替残留物27,30,31和32来创建一系列Nisin A衍生物。我们的研究结果表明，与Nisin A相比，Nisin衍生物在体外表现出降低的止绕抗脉冲活动;然而，在QF样的实验条件下（pH 7 + 22％乳脂），它们都更稳定，特别是H27 / 31K。与Nisin A相比，衍生物H31K和V32K在QF和H27 / 31K中表现出轻微的止血改善，能够将L.单核细胞增生的初始群体减少到最多1.5个log CFU / g。 L.单核细胞元分离物在QF中NISIN暴露的7或14天后表现出对NISIN A或H27 / 31K的类似敏感性。值得注意的是，当与眼糖苷蛋白PLYP100结合时，H27 / 31K的施用在冷却14天后，L.单核细胞生成的不令人令人放量水平。我们的结果突出了生物工程尼本素衍生物的潜力，用于QF中L.单核细胞增强的稳定和增强控制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号