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首页> 外文期刊>Food microbiology >Efficacy of novel phages for control of multi-drug resistant Escherichia coli O177 on artificially contaminated beef and their potential to disrupt biofilm formation
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Efficacy of novel phages for control of multi-drug resistant Escherichia coli O177 on artificially contaminated beef and their potential to disrupt biofilm formation

机译：新噬菌体对人工污染牛肉对多药耐药大肠杆菌O177进行控制的疗效及其扰乱生物膜形成的潜力

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摘要

Contaminated beef is a prominent source of foodborne pathogens such as Escherichia coli O177. Susceptibility of nine multi-drug resistant E. coli O177 strains against eight individual phages and six phage cocktails was assessed using polystyrene microplate titer plate. Further, 180 beef samples were independently inoculated with E. coli O177 cells in triplicates and treated with eight individual phages and six phage cocktails to determine their efficacy in inhibiting bacteria growth at 4 °C over a 7-day incubation period. Results revealed that all E. coli O177 strains were susceptible to the phages. A significant log reduction in viable E. coli O177 cell counts was observed on beef samples upon phage treatment over the 7-day incubation period. Two individual phages and three phage cocktails reduced E. coli cell counts to levels below the detection limit (1.0 log_(10) CFU/g). Log reduction of viable E. coli cell counts ranged from 2.10 to 7.81 CFU/g for individual phages and from 2.86 to 7.81 CFU/g for cocktails. Individual phages and phage cocktails inhibited E. coli 0177 biofilm formation with phage cocktails showing high efficacy. Furthermore, phage cocktails showed greater efficacy in destroying pre-formed biofilm than individual phages. Based on these findings, we concluded that phage cocktails developed in this study could be used to reduce E. coli 0177 contamination and extend the shelf-life of stored raw beef.
机译：污染的牛肉是食物载病原体的突出来源，如大肠杆菌O177。使用聚苯乙烯微孔板滴定板评估九种多药物抗性大肠杆菌O177菌株和六个噬菌体鸡尾酒的敏感性。此外，180个牛肉样品以一式三份，用大肠杆菌O177细胞接种并用8个单独的噬菌体和六个噬菌体鸡尾酒治疗，以确定它们在抑制4℃的细菌生长的疗效7天孵育期。结果表明，所有大肠杆菌O177菌株都易于噬菌体。在7天孵育期间，在噬菌体处理中观察到可行大肠杆菌O177细胞计数的显着降低降低。两种单个噬菌体和三个噬菌体鸡尾酒还原大肠杆菌细胞计数到检测极限以下的水平（1.0 log_（10）Cfu / g）。降低可行的大肠杆菌细胞计数的数量为2.10至7.81 cfu / g，用于各个噬菌体，从2.86到7.81 cfu / g用于鸡尾酒。各个噬菌体和噬菌体鸡尾酒抑制大肠杆菌0177生物膜形成，噬菌体鸡尾酒显示出高疗效。此外，噬菌体鸡尾酒在破坏预形成的生物膜而不是单个噬菌体的疗效表现出更大的疗效。基于这些发现，我们得出结论，该研究中开发的噬菌体鸡尾酒可用于减少大肠杆菌0177污染，并延长储存的原料牛肉的保质期。

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  • 来源
    《Food microbiology》 |2021年第4期|103647.1-103647.10|共10页
  • 作者

    Peter Kotsoana Montso; Victor Mlambo; Collins Njie Ateba;

  • 作者单位

    Food Security and Safety Niche Area Faculty of Natural and Agricultural Sciences North- West University Private Bag X2046 Mmabatho 2735 South Africa;

    School of Agricultural Sciences Faculty of Agriculture and Natural Sciences University of Mpumalanga Private Bag X11283 Mbombela 1200 South Africa;

    Food Security and Safety Niche Area Faculty of Natural and Agricultural Sciences North- West University Private Bag X2046 Mmabatho 2735 South Africa;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Atypical enteropathogenic E. coli; Bio-control; Food safety; Meat; Bacteriophages;

    机译：非典型肠致病大肠杆菌;生物控制;食品安全;肉;噬菌体;

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