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Detection of Fusarium verticillioides by PCR-ELISA based on FUM21 gene

机译:基于FUM21基因的PCR-ELISA检测黄萎病菌

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Fusarium verticillioidesis a primary corn pathogen and fumonisin producer which is associated with toxic effects in humans and animals. The traditional methods for detection of fungal contamination based on morphological characteristics are time-consuming and show low sensitivity and specificity. Therefore, the objective of this study was to develop a PCR-ELISA based on theFUM21gene forF. verticillioidesdetection. The DNA of theF. verticillioides,Fusariumsp.,Aspergillussp. andPenicilliumsp. isolates was analyzed by conventional PCR and PCR-ELISA to determine the specificity. The PCR-ELISA was specific toF. verticillioidesisolates, showed a 2.5 pg detection limit and was 100-fold more sensitive than conventional PCR. In corn samples inoculated withF. verticillioidesconidia, the detection limit of the PCR-ELISA was 1 × 104conidia/g and was also 100-fold more sensitive than conventional PCR. Naturally contaminated corn samples were analyzed by PCR-ELISA based on theFUM21gene and PCR-ELISA absorbance values correlated positively (p < 0.05) withFusariumsp. counts (CFU/g). These results suggest that the PCR-ELISA developed in this study can be useful forF. verticillioidesdetection in corn samples.
机译:枯萎镰刀菌是一种主要的玉米病原体和伏马菌素生产者,与人和动物的毒性有关。传统的基于形态学特征的真菌污染检测方法耗时且灵敏度低和特异性低。因此,本研究的目的是开发基于FUM21基因的PCR-ELISA。检虫体。 F的DNA。 verticillioides,Fusariumsp。,Aspergillussp。和青霉菌通过常规PCR和PCR-ELISA分析分离物以确定特异性。 PCR-ELISA对F具有特异性。拟南芥分离物的检出限为2.5µpg,比常规PCR灵敏度高100倍。在玉米样品中接种F。轮状孢子菌分生孢子,PCR-ELISA的检出限为1××104分孢子/ g,灵敏度也比常规PCR高100倍。基于FUM21基因的PCR-ELISA分析了自然污染的玉米样品,PCR-ELISA吸光度值与Fusariumsp正相关(p <0.05)。计数(CFU / g)。这些结果表明,本研究开发的PCR-ELISA对F可能有用。玉米样品中的类固形物检测。

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