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首页> 外文期刊>Food microbiology >Identification and classification of sampling sites for pathogen environmental monitoring programs for Listeria monocytogenes: Results from an expert elicitation
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Identification and classification of sampling sites for pathogen environmental monitoring programs for Listeria monocytogenes: Results from an expert elicitation

机译：单核细胞增生性李斯特菌病原体环境监测计划的采样点的识别和分类：专家鉴定的结果

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摘要

Pathogen Environmental Monitoring (PEM) programs forListeriaare important to reduce the contamination risk for exposed Ready-To-Eat (RTE) food products withL. monocytogenes. Specific guidance to identify appropriate sampling sites in individual facilities, including equipment and other sites, will facilitate effectiveL. monocytogenescontrol and PEM programs. Key goals ofListeriaPEM programs are to (i) identify and eliminate niches that allow forListeriagrowth and survival and (ii) verify and validate preventive controls such as sanitation programs and sanitation standard operating procedures (SSOPs), sanitary equipment and facility design. Here, an initial list of 77 sampling sites covering Zones 1–4 was assembled based on current literature and guidance documents with initial classification of sites into (i) Zones 1, 2, 3, and 4; (ii) likely niches or transfer sites, and (iii) verification sites or indicator sites. An expert elicitation that included responses from 16 food safety professionals was used to (i) refine sampling site descriptions and identify 6 new sampling sites that were not included in the original list, (ii) refine classification of sites (e.g., into niches versus transfer sites), and (iii) rank sites on level of importance from 1 to 5. The final sample site list includes sampling sites classified by zone and type of site as well as relative importance of site based on reviewer feedback. This document thus provides an initial set of sites that can be used by industry to help in the development or refinement ofListeriaPEM programs. The availability of this ranked list of sampling sites should reduce barriers to development of science basedListeriaPEM programs.
机译：李斯特菌的病原体环境监测（PEM）程序对于降低暴露于L的即食（RTE）食品的污染风险很重要。单核细胞增生在个别设施（包括设备和其他场所）中确定适当采样点的具体指南将有助于有效实施。 monocytogenescontrol和PEM程序。李斯特菌PEM计划的主要目标是（i）识别和消除允许李斯特菌生长和生存的生态位，以及（ii）验证和确认预防性控制措施，例如卫生计划和卫生标准操作程序（SSOP），卫生设备和设施设计。在此，根据当前文献和指导文件汇总了涵盖1至4区的77个采样点的初始列表，并将这些点初步分类为（i）1、2、3和4区； （ii）可能的利基或转移站点，以及（iii）验证站点或指示站点。包括来自16位食品安全专业人员的响应在内的专家启发被用于（i）完善采样地点的描述并确定6个未包括在原始列表中的新采样地点，（ii）完善地点的分类（例如，划分为利基与转移）个站点），以及（iii）将站点的重要性级别从1到5进行排序。最终的样本站点列表包括按区域和站点类型分类的采样站点，以及基于审阅者反馈的站点相对重要性。因此，本文档提供了一组可供行业使用的初始站点，以帮助开发或完善ListeriaPEM程序。该排名的采样点列表的可用性应减少开发基于科学的ListeriaPEM程序的障碍。

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