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首页> 外文期刊>Food microbiology >Variability in gene content and expression of the thioredoxin system in Oenococcus oeni
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Variability in gene content and expression of the thioredoxin system in Oenococcus oeni

机译：Oenococcus oeni基因含量和硫氧还蛋白系统表达的变异性

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摘要

The thioredoxin system protects against oxidative stress through the reversible oxidation of the thioredoxin active center dithiol to a disulphide. The genome of Oenococcus oeni PSU-1 contains three thioredoxin genes (trxA1, trxA2, trxA3), one thioredoxin reductase (trxB) and one ferredoxin reductase (fdr) which, until recently, was annotated as a second thioredoxin reductase. For the first time, the entire thioredoxin system in several O. oeni strains isolated from wine has been analysed. Comparisons at the DNA and protein levels have been undertaken between sequences from O. oeni and other genera and species, and the genera Leuconostoc and Lactobacillus were found to present the highest similarities. The gene most frequently absent from a collection of 34 strains and the sequences annotated in the NCBI database was trxA1. Moreover, phylogenetic analysis suggested that this gene was horizontally transferred from Lactobacillus to O. oeni. Strain-dependent expression profiles were determined in rich and in wine-like media. General over-expression was detected after inoculation into wine-like medium, with trxA3 being the most highly expressed gene. The increased transcriptional levels of the thioredoxin genes are indicative of the crucial role of this system in the O. oeni response to wine harsh conditions.
机译：硫氧还蛋白系统通过将硫氧还蛋白活性中心二硫醇可逆氧化为二硫化物来防止氧化应激。 Oenococcus oeni PSU-1的基因组包含三个硫氧还蛋白基因（trxA1，trxA2，trxA3），一个硫氧还蛋白还原酶（trxB）和一个铁氧还蛋白还原酶（fdr），直到最近才被标注为第二个硫氧还蛋白还原酶。首次分析了从葡萄酒中分离出的几种O. oeni菌株中的整个硫氧还蛋白系统。在O. oeni和其他属和种的序列之间进行了DNA和蛋白质水平的比较，发现Leuconostoc和乳杆菌属具有最高的相似性。收集的34个菌株中最不存在的基因，在NCBI数据库中注释的序列是trxA1。此外，系统发育分析表明该基因从乳酸杆菌水平转移到O. oeni。菌株依赖性表达谱在丰富的和类似酒的培养基中确定。接种到葡萄酒样培养基中后，检测到总体过表达，其中trxA3是表达最强的基因。硫氧还蛋白基因的转录水平增加表明该系统在O. oeni对葡萄酒苛刻条件的反应中起着至关重要的作用。

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  • 来源
    《Food microbiology》 |2017年第2期|23-32|共10页
  • 作者

    Mar Margalef-Catala; Elena Stefanelli; Isabel Araque; Karoline Wagner; Giovanna E. Felis; Albert Bordons; Sandra Torriani; Cristina Reguant;

  • 作者单位

    Departament de Bioquimica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, 1, 43007, Tarragona, Catalonia, Spain;

    Department of Biotechnology, Universita degli Studi di Verona, Verona, Italy;

    Departament de Bioquimica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, 1, 43007, Tarragona, Catalonia, Spain;

    Departament de Bioquimica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, 1, 43007, Tarragona, Catalonia, Spain;

    Department of Biotechnology, Universita degli Studi di Verona, Verona, Italy;

    Departament de Bioquimica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, 1, 43007, Tarragona, Catalonia, Spain;

    Department of Biotechnology, Universita degli Studi di Verona, Verona, Italy;

    Departament de Bioquimica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, 1, 43007, Tarragona, Catalonia, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Oenococcus oeni; Malolactic fermentation; Thioredoxin; Stress; qPCR;

    机译：Oenococcus oeni;苹果乳酸发酵;硫氧还蛋白;强调;定量PCR;

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