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首页> 外文期刊>Food microbiology >Antimicrobial activity of whey protein films supplemented with Lactobacillus sakei cell-free supernatant on fresh beef
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Antimicrobial activity of whey protein films supplemented with Lactobacillus sakei cell-free supernatant on fresh beef

机译：乳清蛋白膜补充清酒乳杆菌无细胞上清液对新鲜牛肉的抗菌活性

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摘要

The aim of this work was to evaluate the antimicrobial activity of whey protein isolate (WPI) films supplemented with Lactobacillus sakei NRRL B-1917 cell-free supernatant on beef inoculated with Escherichia coli ATCC 25922 or Listeria monocytogenes Scott A; additionally, sensory evaluation was performed on wrapped beef cubes. Supernatant concentrates were obtained from Lb. sakei cultures in MRS broth after centrifugation, filtering, and freeze-drying. Films were prepared with WPI (3% w/w), alginate (0.625% w/w), rehydrated supernatant (18 mg/ml), and glycerol. Films were used to wrap beef cubes inoculated with ≈ 10~3 CFU/g E. coli or L. monocytogenes. Sensory evaluation was carried out on grilled beef wrapped or not with the studied antimicrobial films. During refrigerated storage, antimicrobial films reduced 1.4 log_(10) CFU/g of L. monocytogenes after 120 h, while E. coli decreased 2.3 log_(10) CFU/ g after 36 h. Grilled beef wrapped with antimicrobial film was well accepted by panelists, besides scores evidenced no significant differences (p ＞ 0.05) between wrapped and unwrapped beef.
机译：这项工作的目的是评估乳清蛋白分离（WPI）膜补充了日本乳杆菌NRRL B-1917无细胞上清液对接种大肠杆菌ATCC 25922或单核细胞增生李斯特菌Scott A的牛肉的抗菌活性。另外，对包裹的牛肉块进行了感官评估。从Lb获得上清液浓缩物。离心，过滤和冷冻干燥后，在MRS肉汤中添加清酒培养物。用WPI（3％w / w），藻酸盐（0.625％w / w），再水合的上清液（18mg / ml）和甘油制备膜。薄膜用于包裹接种了≈10〜3 CFU / g大肠杆菌或单核细胞增生李斯特菌的牛肉块。感官评估是用研究的抗菌薄膜包裹或不包裹的烤牛肉进行的。在冷藏期间，抗菌膜在120小时后降低单核细胞增生李斯特菌1.4 log_（10）CFU / g，而大肠杆菌在36 h后降低2.3 log_（10）CFU / g。包裹抗菌膜的烤牛肉被小组成员很好地接受，此外，得分也证明包裹和未包裹的牛肉之间没有显着差异（p＞ 0.05）。

著录项

  • 来源
    《Food microbiology》 |2017年第4期|207-211|共5页
  • 作者

    Silvia del Carmen Beristain-Bauza; Emma Mani-Lopez; Enrique Palou; Aurelio Lopez-Malo;

  • 作者单位

    Departamento de Ingenieria Quimica, Alimentos y Ambiental, Universidad de las Americas Pueblo, Cholula, Puebla 72810, Mexico;

    Departamento de Ingenieria Quimica, Alimentos y Ambiental, Universidad de las Americas Pueblo, Cholula, Puebla 72810, Mexico;

    Departamento de Ingenieria Quimica, Alimentos y Ambiental, Universidad de las Americas Pueblo, Cholula, Puebla 72810, Mexico;

    Departamento de Ingenieria Quimica, Alimentos y Ambiental, Universidad de las Americas Pueblo, Cholula, Puebla 72810, Mexico;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Whey protein isolate; Films; Antimicrobial activity; Lactobacillus sakei cell-free supernatant; Beef;

    机译：乳清蛋白分离物;电影;抗菌活性;清酒乳杆菌无细胞上清液;牛肉;

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