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Effect of sub-inhibitory concentrations of biocides on the architecture and viability of MRSA biofilms

机译：亚抑制浓度杀生物剂对MRSA生物膜结构和活力的影响

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摘要

The effect of sub-minimum inhibitory concentrations (sub-MICs) of three biocides (benzalkonium chloride [BZK], trisodium phosphate [TSP] and sodium hypochlorite [SHY]) upon the architecture and viability of the biofilms formed by a methicillin-resistant Staphylococcus aureus strain of food origin (MRSA 48a) was investigated. Images were examined through confocal laser scanning microscopy (CLSM) after staining with SYT09 and propidium iodide. Sub-MICs of BZK or TSP reduced the ability of MRSA to produce biofilm. In contrast, the presence of sub-MICs of SHY enhanced the biofilm-forming ability of MRSA when cells had undergone previous adaptation to this compound (biovolume in the observation field was 137,785.31 ± 47,682.79 μm~3 for biofilms formed in the presence of SHY, and 70,204.13 ± 31,603.98 μm~3 in the absence of biocides; P < 0.05). The largest amount of live (green stained) cells (P < 0.05) was observed in biofilms grown in the presence of SHY relative to the other conditions tested (58,999.75 ± 55,312.37 um~3 vs 31,976.29 ± 38,594.98 μm~3). Findings from the present work constitute the first report of biofilm production by MRSA being induced by sub-inhibitory concentrations of SHY. The data suggest that repeated use of SHY at low concentrations could represent a public health risk.
机译：三种杀生物剂（苯扎氯铵[BZK]，磷酸三钠[TSP]和次氯酸钠[SHY]）的最低抑菌浓度（sub-MICs）对耐甲氧西林的葡萄球菌形成的生物膜的结构和活力的影响研究了食物来源的金黄色葡萄球菌菌株（MRSA 48a）。 SYT09和碘化丙锭染色后，通过共聚焦激光扫描显微镜（CLSM）检查图像。 BZK或TSP的Sub-MIC降低了MRSA产生生物膜的能力。相比之下，当细胞经过先前对该化合物的适应后，SHY亚型MIC的存在增强了MRSA的生物膜形成能力（观察场中的生物体积为137,785.31±47,682.79μm〜3，对于SHY存在时形成的生物膜，在没有杀菌剂的情况下为70,204.13±31,603.98μm〜3; P <0.05）。相对于其他测试条件，在SHY存在下生长的生物膜中观察到最大数量的活（绿色染色）细胞（P <0.05）（58,999.75±55,312.37 um〜3对31,976.29±38,594.98μm〜3）。从目前的工作中发现的结果构成了MRSA被SHY亚抑制浓度诱导产生生物膜的第一份报告。数据表明，重复使用低浓度的SHY可能会构成公共健康风险。

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  • 来源
    《Food microbiology》 |2017年第8期|294-301|共8页
  • 作者

    Laura Buzon-Duran; Carlos Alonso-Calleja; Felix Riesco-Pelaez; Rosa Capita;

  • 作者单位

    Department of Food Hygiene and Technology, University of Leon, E-24071 Leon, Spain, institute of Food Science and Technology, University of Leon, E-24071 Leon, Spain;

    Department of Food Hygiene and Technology, University of Leon, E-24071 Leon, Spain, institute of Food Science and Technology, University of Leon, E-24071 Leon, Spain;

    Department of Electrical Engineering and Systems Engineering and Automatic Control, University of Leon, E-24071 Leon, Spain;

    Department of Food Hygiene and Technology, University of Leon, E-24071 Leon, Spain, institute of Food Science and Technology, University of Leon, E-24071 Leon, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MRSA; Biocides; Biofilm architecture; Biofilm viability; CLSM;

    机译：MRSA;杀菌剂;生物膜结构;生物膜生存力;CLSM;

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