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Effect of food-related stress conditions and loss of agr and sigB on seb promoter activity in S. aureus

机译：食物相关应激条件以及年龄和sigN丧失对金黄色葡萄球菌seb启动子活性的影响

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摘要

Staphylococcal enterotoxin B (SEB) causes staphylococcal food poisoning and is produced in up to ten times higher quantities than other major enterotoxins. While Staphylococcus aureus growth is often repressed by competing flora, the organism exhibits a decisive growth advantage under some stress conditions. So far, data on the influence of food-related stressors and regulatory mutations on seb expression is limited and largely based on laboratory strains, which were later reported to harbor mutations. Therefore, the aim of this study was to investigate the influence of stress and regulatory mutations on seb promoter activity. To this end, transcriptional fusions were created in two strains, USA300 and HG003, carrying different seb upstream sequences fused to a blaZ reporter. NaCl, nitrite, and glucose stress led to significantly decreased seb promoter activity, while lactic acid stress resulted in significantly increased seb promoter activity. Loss of agr decreased seb promoter activity and loss of sigB increased promoter activity, with the magnitude of change depending on the strain. These results demonstrate that mild stress conditions encountered during food production and preservation can induce significant changes in seb promoter activity.
机译：葡萄球菌肠毒素B（SEB）引起葡萄球菌食物中毒，其产生量比其他主要肠毒素高十倍。尽管金黄色葡萄球菌的生长通常会受到竞争菌群的抑制，但该生物体在某些胁迫条件下显示出决定性的生长优势。到目前为止，有关食物相关应激源和调控突变对seb表达影响的数据是有限的，并且主要基于实验室菌株，后来被报道具有突变。因此，本研究的目的是研究压力和调控突变对seb启动子活性的影响。为此，在两个菌株USA300和HG003中产生了转录融合体，它们携带与blaZ报道基因融合的不同的seb上游序列。 NaCl，亚硝酸盐和葡萄糖胁迫导致SEB启动子活性显着降低，而乳​​酸胁迫导致SEB启动子活性显着增加。 agr的损失会降低seb启动子活性，而sigB的损失会增加启动子活性，其变化幅度取决于菌株。这些结果表明，在食品生产和保存过程中遇到的轻度压力条件可以诱导seb启动子活性发生重大变化。

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  • 来源
    《Food microbiology》 |2017年第8期|205-212|共8页
  • 作者

    Henna-Maria Sihto; Roger Stephan; Christoph Engl; John Chenc; Sophia Johler;

  • 作者单位

    Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland;

    Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland;

    Skirball Institute of Biomolecular Medicine, New York University Medical Center New York, New York, USA;

    Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore, Singapore;

    Institute for Food Safety and Hygiene, University of Zurich, Zurich, Switzerland, Skirball Institute of Biomolecular Medicine, New York University Medical Center New York, New York, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Staphylococcus aureus; Staphylococcal enterotoxin B; Stress response; agr; sigB;

    机译：金黄色葡萄球菌;葡萄球菌肠毒素B;压力反应;农业标志;

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